Wavelength-Selective Fluorescence of a Model Transmembrane Peptide: Constrained Dynamics of Interfacial Tryptophan Anchors
Wavelength-Selective Fluorescence of a Model Transmembrane Peptide: Constrained Dynamics of Interfacial Tryptophan Anchors
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模型跨膜肽的波长选择性荧光：界面色氨酸锚的约束动力学
DOI：
10.1007/s10895-018-2293-5
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发表时间：
2018
影响因子：
2.7
通讯作者：
Chattopadhyay, Amitabha
中科院分区：
文献类型：
--
作者：
Pal, Sreetama;Koeppe, Roger E.;Chattopadhyay, Amitabha
WALP是典型的α-螺旋跨膜肽，代表了完整膜蛋白跨膜片段的共同序列，是研究膜中肽-脂相互作用和疏水错配的优秀模型。重要的是，Walp肽与脂质直接接触。它们由中央延伸的交替的疏水性丙氨酸和亮氨酸残基组成，两端由色氨酸覆盖。在这项工作中，我们使用波长选择荧光方法来探索1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine(POPC)膜中WALP23中色氨酸残基的本征荧光。结果表明，WALP23中的四个色氨酸残基的平均红边激发位移(REES)为6 nm，表明它们定位于膜界面，具有受限的微环境特征。这一结果得到了POPC膜中WALP23色氨酸显示的荧光各向异性和寿命随波长变化的测量结果的支持。这些结果提供了一种基于界面色氨酸的本征荧光来解决蛋白质-脂类相互作用和疏水失配的新方法。
WALPs are prototypical, α-helical transmembrane peptides that represent a consensus sequence for transmembrane segments of integral membrane proteins and serve as excellent models for exploring peptide-lipid interactions and hydrophobic mismatch in membranes. Importantly, the WALP peptides are in direct contact with the lipids. They consist of a central stretch of alternating hydrophobic alanine and leucine residues capped at both ends by tryptophans. In this work, we employ wavelength-selective fluorescence approaches to explore the intrinsic fluorescence of tryptophan residues in WALP23 in 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. Our results show that the four tryptophan residues in WALP23 exhibit an average red edge excitation shift (REES) of 6 nm, implying their localization at the membrane interface, characterized by a restricted microenvironment. This result is supported by fluorescence anisotropy and lifetime measurements as a function of wavelength displayed by WALP23 tryptophans in POPC membranes. These results provide a new approach based on intrinsic fluorescence of interfacial tryptophans to address protein-lipid interaction and hydrophobic mismatch.
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影响因子：
2.9
作者：
Killian, JA;Salemink, I;Greathouse, DV
通讯作者：
Greathouse, DV
pHLIP 在低浓度水溶液中的聚集行为：荧光研究
影响因子：
2.7
作者：
B. D. Rao;H. Chakraborty;S. Keller;A. Chattopadhyay
通讯作者：
A. Chattopadhyay
影响因子：
2
作者：
CHEN, GC;YANG, JT
通讯作者：
YANG, JT
肽/脂质疏水性不匹配对模拟大肠杆菌膜脂质组成的模型膜相行为的影响。
影响因子：
3.4
作者：
S. Morein;R. E. Koeppe II;G. Lindblom;B. de Kruijff;J. Killian
通讯作者：
J. Killian
影响因子：
3.4
作者：
Chaudhuri, Arunima;Haldar, Sourav;Sun, Haiyan;Koeppe, Roger E., II;Chattopadhyay, Amitabha
通讯作者：
Chattopadhyay, Amitabha