Automated analysis of NeuronJ tracing data.
Automated analysis of NeuronJ tracing data.
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DOI：
10.1002/cyto.a.20660
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发表时间：
2009-04
期刊：
影响因子：
3.7
通讯作者：
Lanier, Lorene M.
中科院分区：
文献类型：
--
作者：
Popko, Jonathan;Fernandes, Adelaide;Brites, Dora;Lanier, Lorene M.
神经细胞体外分化的研究通常涉及对神经突起的追踪和分析。NeuronJ是一个可用于对单个神经元进行半自动跟踪的程序；当跟踪完成时，会生成一个包含神经突起长度测量的文本文件。利用培养的海马神经元，我们发现，要达到统计学意义，通常需要追踪每个处理组中约100个神经元。后期跟踪数据分析需要将每个文本文件导入统计程序。分析不同的参数，如治疗对轴突和树突分支的影响，需要大量耗时的追踪后数据处理。我们已经开发了XL_Calculations，这是一个基于Java的程序，可以对NeuronJ测量文件执行批处理分析，并自动进行多项计算，包括轴突和树突上的一级、二级和三级轴突的数量、长度和总输出(总和长度)，并将计算写入Excel工作表。批量处理NeuronJ测量文件大大减少了分析神经元形态所需的时间。此外，我们的程序执行超过45种不同的计算，能够详细确定治疗对神经元分化的影响。使用这个程序分析NeuronJ追踪数据，我们证明了持续地将分化的海马神经元暴露于Netrin 1增加了轴突和树突上的次级分支的数量，而不显著改变轴突、树突或分支的长度。无论神经元生长在聚D-赖氨酸还是层粘连蛋白上，都得到了类似的结果。
Studies of neuronal differentiation in vitro often involve tracing and analysis of neurites. NeuronJ is a program that can be used for semi-automated tracing of individual neurons; when tracing is completed, a text file containing neurite length measurements is generated. Using cultured hippocampal neurons, we have found that to reach statistical significance it is generally necessary to trace about 100 neurons in each treatment group. Post tracing data analysis requires importing each text file into a statistics program. Analysis of distinct parameters, such as effects of a treatment on axonal versus dendritic branching, requires a great deal of time consuming post tracing data manipulation. We have developed XL_Calculations, a Java based program that performs batch analysis on NeuronJ measurement files and automatically makes multiple calculations, including the number, length and total output (sum length) of primary, secondary and tertiary neurites on axons and dendrites and writes the calculations into an Excel worksheet. Batch processing of NeuronJ measurement files dramatically reduces the time required to analyze neuronal morphology. In addition, our program performs more than 45 distinct calculations, enabling detailed determination of treatment effects on neuronal differentiation. Using this program to analyze NeuronJ tracing data, we demonstrate that continuous exposure of differentiating hippocampal neurons to Netrin 1 increases the number of secondary branches on both axons and dendrites, without significantly altering the length of the axon, dendrites or branches. Similar results were obtained whether neurons were grown on poly-d-lysine or laminin.
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影响因子：
16.2
作者：
Lanier, LM;Gates, MA;Gertler, FB
通讯作者：
Gertler, FB
影响因子：
5.3
作者：
Dent, EW;Barnes, AM;Kalil, K
通讯作者：
Kalil, K
DOI：
10.1016/0165-3806(92)90159-t
发表时间：
1992-10-23
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
LEIN, PJ;BANKER, GA;HIGGINS, D
通讯作者：
HIGGINS, D
影响因子：
3.7
作者：
Meijering, E;Jacob, M;Unser, M
通讯作者：
Unser, M
影响因子：
21.3
作者：
Dent, Erik W.;Kwiatkowski, Adam V.;Gertler, Frank B.
通讯作者：
Gertler, Frank B.