Genome-Wide Analysis of APETALA2/Ethylene-Responsive Factor (AP2/ERF) Gene Family in Barley (Hordeum vulgare L.).

Genome-Wide Analysis of APETALA2/Ethylene-Responsive Factor (AP2/ERF) Gene Family in Barley (Hordeum vulgare L.).
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大麦 (Hordeum vulgare L.) 中 APETALA2/乙烯反应因子 (AP2/ERF) 基因家族的全基因组分析

DOI:
10.1371/journal.pone.0161322
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发表时间:
2016
期刊:
影响因子:
3.7
通讯作者:
Xu R
Xu R
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Guo B;Wei Y;Xu R;Lin S;Luan H;Lv C;Zhang X;Song X;Xu R

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APETALA 2/ERF(Ethylene-Responsive Factor)基因家族是植物特异性转录因子。它在植物的发育过程、对生物和非生物胁迫的耐受性以及对激素的反应中起着关键作用。然而,有关大麦中AP 2/ERF基因家族的贡献的数据有限。本研究利用生物信息学方法,从大麦中克隆了121个HvAP 2/ERF基因。HvAP 2/ERF基因共定位118个(97.5%),分布在7条染色体上。根据拟南芥AP 2/ERF家族的系统发育分类,HvAP 2/ERF蛋白可分为AP 2(APETALA 2)、RAV(Related to ABI 3/VP)、DREB(dehydration responsive element binding)、ERF(ethylene responsive factors)和soloist亚家族。重复事件的分析表明,串联重复和片段重复有助于扩大的AP 2/ERF家族在大麦。HvDREB 1 s/2s基因在非生物胁迫和激素胁迫下表现出不同的表达模式。总之,本研究中产生的数据将有助于全基因组分析,以确定HvAP 2/ERF基因在大麦发育,非生物胁迫和植物激素反应中的确切作用,最终目标是提高作物产量。
APETALA2/Ethylene-Responsive Factor (AP2/ERF) gene family is plant specific transcription factor. It plays critical roles in development process, tolerance to biotic and abiotic stresses, and responses to plant hormones. However, limited data are available on the contributions of AP2/ERF gene family in barley (Hordeum vulgare L.). In the present study, 121 HvAP2/ERF genes in barley were identified by using bioinformatics methods. A total of 118 HvAP2/ERF (97.5%) genes were located on seven chromosomes. According to phylogenetic classification of AP2/ERF family in Arabidopsis, HvAP2/ERF proteins were divided into AP2 (APETALA2), RAV (Related to ABI3/VP), DREB (dehydration responsive element binding), ERF (ethylene responsive factors) and soloist sub families. The analysis of duplication events indicated that tandem repeat and segmental duplication contributed to the expansion of the AP2/ERF family in barley. HvDREB1s/2s genes displayed various expression patterns under abiotic stress and phytohormone. Taken together, the data generated in this study will be useful for genome-wide analysis to determine the precise role of the HvAP2/ERF gene during barley development, abiotic stress and phytohormone responses with the ultimate goal of improving crop production.
DOI: 10.1371/journal.pone.0118503
发表时间: 2015
期刊: PloS one
影响因子: 3.7
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