Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations.
Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations.
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DOI：
10.1093/nar/gkt1329
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发表时间：
2014-04
影响因子：
14.9
通讯作者：
SantaLucia J Jr
中科院分区：
文献类型：
--
作者：
Jiang J;Aduri R;Chow CS;SantaLucia J Jr
H69是核糖体大亚基RNA的一个19-nt的茎环区。在H69中聚集的三个假尿苷（pseudouridine，pseudouridine）修饰在整个遗传学中是保守的，并且已知影响核糖体功能。为了探讨β-内酰胺酶对大肠杆菌H69在溶液中构象的影响，采用核磁共振波谱法研究了β-内酰胺酶修饰前后H69的结构差异。两种结构的比较表明，H69双链可变区具有以下独特的特征：（i）环区由A1911和A1919之间的Watson-Crick碱基对封闭，这可能通过涉及A1911 N1 H的相互作用而得到加强;（ii）环残基1915和1917处的双链可变区修饰促进了A1915至A1918的碱基堆叠。相比之下，H69 UUU环区，其缺乏修饰，组织性较差。结构调节由H69环区域的5'一半的构象行为的改变，观察到的C1914不可交换的碱基质子共振在H69环核磁共振谱加宽，并在建立核糖体亚基间桥B2 a和介导翻译保真度中发挥重要的生物学作用。
Helix 69 (H69) is a 19-nt stem-loop region from the large subunit ribosomal RNA. Three pseudouridine (Ψ) modifications clustered in H69 are conserved across phylogeny and known to affect ribosome function. To explore the effects of Ψ on the conformations of Escherichia coli H69 in solution, nuclear magnetic resonance spectroscopy was used to reveal the structural differences between H69 with (ΨΨΨ) and without (UUU) Ψ modifications. Comparison of the two structures shows that H69 ΨΨΨ has the following unique features: (i) the loop region is closed by a Watson–Crick base pair between Ψ1911 and A1919, which is potentially reinforced by interactions involving Ψ1911N1H and (ii) Ψ modifications at loop residues 1915 and 1917 promote base stacking from Ψ1915 to A1918. In contrast, the H69 UUU loop region, which lacks Ψ modifications, is less organized. Structure modulation by Ψ leads to alteration in conformational behavior of the 5' half of the H69 loop region, observed as broadening of C1914 non-exchangeable base proton resonances in the H69 ΨΨΨ nuclear magnetic resonance spectra, and plays an important biological role in establishing the ribosomal intersubunit bridge B2a and mediating translational fidelity.
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DOI：
10.1073/pnas.0607541103
发表时间：
2006-10-24
影响因子：
11.1
作者：
Berk, Veysel;Zhang, Wen;Cate, Jamie H. Doudna
通讯作者：
Cate, Jamie H. Doudna
影响因子：
5.5
作者：
Aduri, Raviprasad;Psciuk, Brian T.;SantaLucia, John, Jr.
通讯作者：
SantaLucia, John, Jr.
影响因子：
14.9
作者：
Davis, DR
通讯作者：
Davis, DR
影响因子：
2.9
作者：
HALL, KB;MCLAUGHLIN, LW
通讯作者：
MCLAUGHLIN, LW
DOI：
10.1107/s0907444998003254
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Brunger, AT;Adams, PD;Warren, GL
通讯作者：
Warren, GL