Immunologically augmented skin flap as a novel dendritic cell vaccine against head and neck cancer in a rat model.
Immunologically augmented skin flap as a novel dendritic cell vaccine against head and neck cancer in a rat model.
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DOI：
10.1111/cas.12586
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发表时间：
2015-02
期刊：
影响因子：
5.7
通讯作者：
Akiyama Y
中科院分区：
文献类型：
--
作者：
Inoue K;Saegusa N;Omiya M;Ashizawa T;Miyata H;Komiyama M;Iizuka A;Kume A;Sugino T;Yamaguchi K;Kiyohara Y;Nakagawa M;Akiyama Y
局部复发是头颈部肿瘤手术切除后的主要临床问题，由于缺乏合适的治疗方法，微小残留病的播散和淋巴结转移相对难以治疗。在本研究中，我们开发并评估了一种在大鼠肿瘤模型中使用致敏树突状细胞（DC）处理的皮瓣转移的新型免疫疗法，称为“免疫皮瓣”。局部切除皮肤圆形区域后，将SCC-158细胞（一种大鼠头颈部癌细胞系）接种于肌肉表面；最后将注入成熟DC的腹股沟皮瓣进行复盖。第二次DC注射2周后，测量全身免疫反应和肿瘤大小。与对照组相比，dc治疗组的肿瘤大小明显减小。虽然脾细胞CTL活性的诱导是微弱的，但在dc治疗组，Th1细胞因子如白细胞介素-2和干扰素-γ升高。这些结果提示，一种基于免疫皮瓣的新型免疫治疗方法在预防头颈癌患者局部复发方面具有临床应用潜力。
Local recurrence is a major clinical issue following surgical resection in head and neck cancer, and the dissemination and lymph node metastasis of minimal residual disease is relatively difficult to treat due to the lack of suitable therapeutic approaches. In the present study, we developed and evaluated a novel immunotherapy using a skin flap transfer treated with sensitized dendritic cells (DC), termed the “immuno-flap,” in a rat tumor model. After the local round area of skin was resected, SCC-158 cells (a rat head and neck cancer cell line) were inoculated into the muscle surface; lastly, the groin skin flap injected with mature DC was overlaid. Two weeks after the second DC injection, systemic immunological reactions and tumor size were measured. The DC-treated group showed a significant reduction in tumor size compared with the control. Although the induction of CTL activity in spleen cells was marginal, Th1 cytokines such as interleukin-2 and interferon-γ were elevated in the DC-treated group. These results suggest that a novel immunotherapy based on the immuno-flap method has the potential for clinical application to prevent the local recurrence of head and neck cancer patients.
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影响因子：
5.8
作者：
Uchida, Tetsuro;Horiguchi, Shigetoshi;Okamoto, Yoshitaka
通讯作者：
Okamoto, Yoshitaka
癌症干细胞在头部和颈部鳞状细胞癌的进展中问责制：最近的趋势！
DOI：
10.1155/2014/375325
发表时间：
2014
期刊：
Molecular biology international
影响因子：
--
作者：
Routray S;Mohanty N
通讯作者：
Mohanty N
影响因子：
3.1
作者：
Christian, Natalie A.;Benencia, Fabian;Milone, Michael C.;Li, Guizhi;Frail, Paul R.;Therien, Michael J.;Coukos, George;Hammer, Daniel A.
通讯作者：
Hammer, Daniel A.
影响因子：
11.5
作者：
Aarntzen, Erik H. J. G.;Srinivas, Mangala;de Vries, I. Jolanda M.
通讯作者：
de Vries, I. Jolanda M.
影响因子：
3.3
作者：
Kornfehl, J;Formanek, M;Millesi, W
通讯作者：
Millesi, W