Comparative analysis of the Photorhabdus luminescens and the Yersinia enterocolitica genomes: uncovering candidate genes involved in insect pathogenicity.
Comparative analysis of the Photorhabdus luminescens and the Yersinia enterocolitica genomes: uncovering candidate genes involved in insect pathogenicity.
复制标题
DOI：
10.1186/1471-2164-9-40
复制
发表时间：
2008-01-25
期刊：
影响因子：
4.4
通讯作者：
Fuchs TM
中科院分区：
文献类型：
--
作者：
Heermann R;Fuchs TM
发光杆菌和小肠结肠炎耶尔森菌都是与昆虫相关的肠道细菌。发光假单胞菌与土壤线虫共生，对昆虫具有高致病性，但对人类无致病性。相比之下，小肠结肠炎耶尔森氏菌广泛存在于环境中，已知主要会引起男性胃肠炎，但直到最近才被证明对昆虫也有毒。预计这两种病原体在昆虫宿主中发挥作用的遗传决定因素有重叠。应用选择性基因组比较。已经分析了属于双组分调节系统、群体感应、通用应激蛋白和 c-di-GMP 信号传导类的蛋白质。选定的调节系统的有机间概要揭示了两种病原体用于昆虫宿主内信号感知的共同和独特的信号机制。特别是，鉴定出一类新的类似 LuxR 的调节因子，它可能参与检测昆虫特异性分子。此外，遗传重叠揭示了光杆菌属和耶尔森氏菌属所特有的双组分系统，因此被认为在病原体-昆虫关系中发挥着重要作用。我们的分析还强调了这两种病原体在昆虫中遇到的低温下表达的因素，而不是较高（体温）的温度，这提供了证据表明温度是细菌适应各种宿主的一个尚未得到充分研究的环境信号。描述了常见的降解代谢途径，可用于探索昆虫肠道或血淋巴内的营养物质，从而使发光假单胞菌和小肠结肠炎耶尔森氏菌在其无脊椎动物宿主中增殖。与小肠结肠炎耶尔森氏菌相比，发光假单胞菌中编码杀虫毒素和其他毒力因子的基因数量显着增加，这与发光假单胞菌对昆虫的更高毒力相关，并表明该病原体具有更广泛的昆虫宿主谱。确定了两种病原体共有的一组因素，包括与宿主感染过程、昆虫体内持续存在或宿主利用有关的因素。其中一些可能是在与昆虫交往过程中被选择的，然后适应哺乳动物宿主的发病机制。
Photorhabdus luminescens and Yersinia enterocolitica are both enteric bacteria which are associated with insects. P. luminescens lives in symbiosis with soil nematodes and is highly pathogenic towards insects but not to humans. In contrast, Y. enterocolitica is widely found in the environment and mainly known to cause gastroenteritis in men, but has only recently been shown to be also toxic for insects. It is expected that both pathogens share an overlap of genetic determinants that play a role within the insect host. A selective genome comparison was applied. Proteins belonging to the class of two-component regulatory systems, quorum sensing, universal stress proteins, and c-di-GMP signalling have been analysed. The interorganismic synopsis of selected regulatory systems uncovered common and distinct signalling mechanisms of both pathogens used for perception of signals within the insect host. Particularly, a new class of LuxR-like regulators was identified, which might be involved in detecting insect-specific molecules. In addition, the genetic overlap unravelled a two-component system that is unique for the genera Photorhabdus and Yersinia and is therefore suggested to play a major role in the pathogen-insect relationship. Our analysis also highlights factors of both pathogens that are expressed at low temperatures as encountered in insects in contrast to higher (body) temperature, providing evidence that temperature is a yet under-investigated environmental signal for bacterial adaptation to various hosts. Common degradative metabolic pathways are described that might be used to explore nutrients within the insect gut or hemolymph, thus enabling the proliferation of P. luminescens and Y. enterocolitica in their invertebrate hosts. A strikingly higher number of genes encoding insecticidal toxins and other virulence factors in P. luminescens compared to Y. enterocolitica correlates with the higher virulence of P. luminescens towards insects, and suggests a putative broader insect host spectrum of this pathogen. A set of factors shared by the two pathogens was identified including those that are involved in the host infection process, in persistence within the insect, or in host exploitation. Some of them might have been selected during the association with insects and then adapted to pathogenesis in mammalian hosts.
登录
查看更多内容
影响因子：
3.2
作者：
Bresolin, G;Neuhaus, K;Fuchs, TM
通讯作者：
Fuchs, TM
DOI：
10.1046/j.1432-1033.2002.02978.x
发表时间：
2002-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Bochkareva, ES;Girshovich, AS;Bibi, E
通讯作者：
Bibi, E
影响因子：
7.7
作者：
Basset, A;Tzou, P;Boccard, F
通讯作者：
Boccard, F
影响因子：
3.2
作者：
Atkinson, S;Chang, CY;Williams, P
通讯作者：
Williams, P
影响因子：
3.6
作者：
Camilli, A;Mekalanos, JJ
通讯作者：
Mekalanos, JJ