Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43.
Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43.
复制标题
棉酚通过降低 Connexin43 的表达来抑制支持细胞之间的间隙连接细胞间通讯。
DOI：
10.1016/j.tiv.2008.07.012
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发表时间：
2008-10
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
先前的研究表明，棉酚可以阻断培养细胞之间的间隙连接细胞间通讯（GJIC）。本研究旨在探讨棉酚对培养细胞中 GJIC 和连接蛋白 43 (Cx43) 表达的影响。将支持细胞系TM4用不同浓度的棉酚1.25、2.5、5和10μmol/L处理6、12、24和48小时。使用 CCK-8 测定评估细胞活力。使用刮擦负载和染料转移（SLDT）测定法测定细胞中的 GJIC；通过RT-PCR、免疫荧光和Western blot分析检测Cx43的表达。 SLDT 测定显示棉酚显着降低相邻细胞之间的 GJIC。 RT-PCR、免疫荧光和蛋白质印迹分析证明了 TM4 细胞中 Cx43 的表达。随着棉酚浓度的增加，Cx43的表达逐渐降低，这种效应早在处理后6小时就出现，一直持续到48小时。这些结果表明，棉酚通过降低细胞中的 Cx43 表达而损害 GJIC，这对于支持细胞调节精子发生很重要。
Previous studies showed that gossypol could block the gap junctional intercellular communication (GJIC) between cultured cells. The present study was designed to investigate the effects of gossypol on the GJIC and the expression of connexin43 (Cx43) in the cultured cells. A Sertoli cell line, TM4, was treated with different concentrations of gossypol 1.25, 2.5, 5, and 10μmol/L for 6, 12, 24, and 48h. Cell viability was assessed with CCK-8 assay. GJIC in the cells was determined using the scrape loading and dye transfer (SLDT) assay; the expression of Cx43 was detected by RT-PCR, immunofluorescence and Western blot analysis. The SLDT assay showed gossypol significantly decreased GJIC between adjacent cells. RT-PCR, immunofluorescence and Western blot analyses demonstrated the expression of Cx43 in TM4 cells. The expression of Cx43 was gradually decreased with the increasing concentrations of gossypol, and the effect occurred as early as 6h after the treatment and continued until 48h. These results suggested that gossypol impaired GJIC by decrease of Cx43 expression in the cells, which is important for Sertoli cells to regulate spermatogenesis.
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DOI：
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发表时间：
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期刊：
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影响因子：
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