LncRNA TMPO-AS1 serves as a sponge for miR-4731-5p modulating breast cancer progression through FOXM1
LncRNA TMPO-AS1 serves as a sponge for miR-4731-5p modulating breast cancer progression through FOXM1
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LncRNA TMPO-AS1 作为 miR-4731-5p 的海绵，通过 FOXM1 调节乳腺癌进展
DOI：
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发表时间：
2021
影响因子：
2.2
通讯作者：
Jiang Guoqin
中科院分区：
文献类型：
--
作者：
Wang Ying;Ma Jun;Li Ruiqing;Gao Xiaokang;Wang Hongli;Jiang Guoqin
目的：研究IncRNA-TMPO-AS 1在乳腺癌中的作用，并探讨其分子机制。方法：采用qRT-PCR方法对TMPO-AS 1、miR-4731- 5 p和FOXM 1进行定量检测。采用CCK-8法、平板克隆形成实验、创伤愈合实验、Transwell实验和流式细胞术等方法对BC细胞的生物学行为进行评价。双荧光素酶报告基因检测用于评估TMPO-AS 1与其下游靶标之间的相互作用。Western blot法检测细胞凋亡和细胞周期相关蛋白的表达。结果：在乳腺癌组织和细胞中，TMPO-AS 1表达明显增加（P<0.05）。功能研究表明，TMPO AS 1基因的敲低可显著抑制肿瘤细胞的生长和迁移（P<0.05）。在机制上，TMPO-AS 1负调控miR-4731- 5 p并通过miR-4731- 5 p/FOXM 1轴影响BC的进展。结论：LncRNA TMPO-AS 1介导miR-4731- 5 p通过FOXM 1调控BC进展。
Objective: To investigate the function of IncRNA-TMPO-AS1 in breast cancer (BC) and to further explore its molecular mechanism. Methods: TMPO-AS1, miR-4731-5p and FOXM1 were quantitatively determined using qRT-PCR. CCK-8 assays, plate cloning experiments, wound healing and Transwell assays, and flow cytometry were used to assess the biological behaviors of BC cells. Dual-luciferase reporter assays were used to assess the interactions between TMPO-AS1 and its downstream targets. The apoptosis and cell cycle-related proteins were quantitatively determined using Western blot. Results: In the BC tissues and cells, TMPO-AS1 was significantly increased (P<0.05). Functional studies suggest that the knockdown of TMPO-AS1 tremendously restrains tumor cell growth and migration (P<0.05). Mechanically, TMPO-AS1 negatively regulates miR-4731-5p and influences the progression of BC through the miR-4731-5p/FOXM1 axis. Conclusion: LncRNA TMPO-AS1spongess miR-4731-5p to modulate BC progression through FOXM1.
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影响因子：
3.7
作者：
Wang B;Wang H;Yang Z
通讯作者：
Yang Z
DOI：
10.1007/s00018-017-2626-6
发表时间：
2018-03
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Yamamura S;Imai-Sumida M;Tanaka Y;Dahiya R
通讯作者：
Dahiya R
影响因子：
11.2
作者：
M. Teh;Soon-Tee Wong;G. Neill;L. Ghali;M. Philpott;A. Quinn
通讯作者：
M. Teh;Soon-Tee Wong;G. Neill;L. Ghali;M. Philpott;A. Quinn
DOI：
10.1126/science.1240925
发表时间：
2013-08-16
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Carpenter S;Aiello D;Atianand MK;Ricci EP;Gandhi P;Hall LL;Byron M;Monks B;Henry-Bezy M;Lawrence JB;O'Neill LA;Moore MJ;Caffrey DR;Fitzgerald KA
通讯作者：
Fitzgerald KA
影响因子：
4
作者：
Cui, Huaan;Zhao, Jiang
通讯作者：
Zhao, Jiang