Genomic diversity of human T-lymphotropic virus type III (HTLV-III).
Genomic diversity of human T-lymphotropic virus type III (HTLV-III).
复制标题
人类嗜 T 淋巴细胞病毒 III 型 (HTLV-III) 的基因组多样性。
作者：
F. Wong;G. Shaw;B. Hahn;S. Salahuddin;M. Popovič;P. Markham;R. Redfield;R. Gallo
从18名艾滋病患者或艾滋病高危人群中分离的人t淋巴细胞嗜型病毒（HTLV-III）的DNA基因组进行了评估，以寻找变异的证据。虽然所有18个病毒DNA在其整个基因组中杂交到原始HTLV-III分离物的全长克隆探针，但18个分离物中的每一个都显示出不同的限制性内切酶模式。分离株间限制性内切位点的差异从23株的1个位点到31株的至少16个位点不等。没有特定的病毒基因型与特定的疾病状态相关，18例患者中有2例有证据表明同时感染了一种以上的病毒基因型。三种不同的病毒分离株在体外繁殖长达9个月的时间里，它们的酶切模式没有发生可检测到的变化。这些发现表明，HTLV-III的不同分离株包括一系列高度相关但可区分的病毒，这对HTLV-III的致病性和开发有效的诊断、治疗和预防措施具有重要意义。
The DNA genomes of human T-lymphotropic virus type III (HTLV-III) isolated from 18 individuals with AIDS or who were at risk for AIDS were evaluated for evidence of variation. Although all of the 18 viral DNA's hybridized throughout their entire genomes to a full-length cloned probe of the original HTLV-III isolate, each of the 18 isolates showed a different restriction enzyme pattern. The number of restriction site differences between isolates ranged from only 1 site in 23 to at least 16 sites in 31. No particular viral genotype was associated with a particular disease state and 2 of the 18 patients had evidence of concurrent infection by more than one viral genotype. Propagation of three different viral isolates in vitro for up to 9 months did not lead to detectable changes in their restriction patterns. These findings indicate that different isolates of HTLV-III comprise a spectrum of highly related but distinguishable viruses and have important implications regarding the pathogenicity of HTLV-III and attempts to develop effective diagnostic, therapeutic, and preventive measures for this virus.
影响因子：
56.9
作者：
GONDA, MA;WONGSTAAL, F;GILDEN, RV
通讯作者：
GILDEN, RV