Rapid detection of MERS coronavirus-like viruses in bats: pote1ntial for tracking MERS coronavirus transmission and animal origin.
Rapid detection of MERS coronavirus-like viruses in bats: pote1ntial for tracking MERS coronavirus transmission and animal origin.
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DOI：
10.1038/s41426-017-0016-7
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发表时间：
2018-03-07
影响因子：
13.2
通讯作者：
Yuen KY
中科院分区：
文献类型：
--
作者：
Woo PCY;Lau SKP;Chen Y;Wong EYM;Chan KH;Chen H;Zhang L;Xia N;Yuen KY
最近，我们开发了一种基于单克隆抗体的快速核衣壳蛋白检测方法，用于诊断人类和单峰骆驼中的MERS冠状病毒（MERS-CoV）。在这项研究中，我们研究了这种检测方法在蝙蝠中检测与MERS-CoV密切相关的其他谱系C β冠状病毒的有用性。MERS-CoV核衣壳蛋白快速检测方法在27份厚足泰勒蝠（Tylonycteris pachypus）食物样本中检测到24份（88.9%）为阳性，在21份伏翼（Pipistrellus abramus）食物样本中检测到4份（19.0%）为阳性。通过快速MERS-CoV核衣壳蛋白检测试验检测为阳性的Ty-BatCoV-HKU 4 RNA阳性食物样品显著多于Pi-BatCoV-HKU 5 RNA阳性食物样品（通过卡方检验P < 0.001）。在所有51份食源性冠状病毒RNA阳性（菊头蝠冠状病毒HKU 2、鼠耳蝠冠状病毒HKU 6、长翼蝠冠状病毒HKU 8和蹄蝠冠状病毒HKU 10）和32份食源性乙型冠状病毒谱系B（SARS相关菊头蝠冠状病毒HKU 3）和谱系D（Rousettus蝙蝠冠状病毒HKU 9）阳性的食源性样本中，快速检测均为阴性。在通过快速检测（Mann-Witney U检测）检测为阳性和阴性的Ty-BatCoV-HKU 4/Pi-BatCoV-HKU 5 RNA阳性消化道样本的病毒载量之间未观察到显著差异。MERS-CoV核衣壳蛋白快速检测试验能够快速检测蝙蝠中的谱系C β冠状病毒。它检测到的Ty-BatCoV-HKU 4显著多于Pi-BatCoV-HKU 5，因为MERS-CoV与Ty-BatCoV-HKU 4的关系比Pi-BatCoV-HKU 5更密切。这种检测方法将有助于对MERS CoV的祖先动物样本进行快速现场大规模筛查，并跟踪相关蝙蝠物种的传播。
Recently, we developed a monoclonal antibody-based rapid nucleocapsid protein detection assay for diagnosis of MERS coronavirus (MERS-CoV) in humans and dromedary camels. In this study, we examined the usefulness of this assay to detect other lineage C betacoronaviruses closely related to MERS-CoV in bats. The rapid MERS-CoV nucleocapsid protein detection assay was tested positive in 24 (88.9%) of 27 Tylonycteris bat CoV HKU4 (Ty-BatCoV-HKU4) RNA-positive alimentary samples of Tylonycteris pachypus and 4 (19.0%) of 21 Pipistrellus bat CoV HKU5 (Pi-BatCoV-HKU5) RNA-positive alimentary samples of Pipistrellus abramus. There was significantly more Ty-BatCoV-HKU4 RNA-positive alimentary samples than Pi-BatCoV-HKU5 RNA-positive alimentary samples that were tested positive by the rapid MERS-CoV nucleocapsid protein detection assay (P < 0.001 by Chi-square test). The rapid assay was tested negative in all 51 alimentary samples RNA-positive for alphacoronaviruses (Rhinolophus bat CoV HKU2, Myotis bat CoV HKU6, Miniopterus bat CoV HKU8 and Hipposideros batCoV HKU10) and 32 alimentary samples positive for lineage B (SARS-related Rhinolophus bat CoV HKU3) and lineage D (Rousettus bat CoV HKU9) betacoronaviruses. No significant difference was observed between the viral loads of Ty-BatCoV-HKU4/Pi-BatCoV-HKU5 RNA-positive alimentary samples that were tested positive and negative by the rapid test (Mann-Witney U test). The rapid MERS-CoV nucleocapsid protein detection assay is able to rapidly detect lineage C betacoronaviruses in bats. It detected significantly more Ty-BatCoV-HKU4 than Pi-BatCoV-HKU5 because MERS-CoV is more closely related to Ty-BatCoV-HKU4 than Pi-BatCoV-HKU5. This assay will facilitate rapid on-site mass screening of animal samples for ancestors of MERS-CoV and tracking transmission in the related bat species.
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影响因子：
3.7
作者：
Woo PC;Lau SK;Li KS;Poon RW;Wong BH;Tsoi HW;Yip BC;Huang Y;Chan KH;Yuen KY
通讯作者：
Yuen KY
影响因子：
13.2
作者：
Chen, Yixin;Chan, Kwok-Hung;Woo, Patrick C. Y.
通讯作者：
Woo, Patrick C. Y.
影响因子：
11.8
作者：
Wernery, Ulrich;Corman, Victor M.;Drosten, Christian
通讯作者：
Drosten, Christian
影响因子：
6.4
作者：
Alagaili, Abdulaziz N.;Briese, Thomas;Lipkin, W. Ian
通讯作者：
Lipkin, W. Ian
影响因子：
6.4
作者：
van Boheemen S;de Graaf M;Lauber C;Bestebroer TM;Raj VS;Zaki AM;Osterhaus AD;Haagmans BL;Gorbalenya AE;Snijder EJ;Fouchier RA
通讯作者：
Fouchier RA