Molecular diversity of coronaviruses in bats.

Molecular diversity of coronaviruses in bats.
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DOI:
10.1016/j.virol.2006.02.041
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发表时间:
2006-07-20
期刊:
影响因子:
3.7
通讯作者:
Yuen KY
Yuen KY
中科院分区:
医学3区
文献类型:
--
作者:
Woo PC;Lau SK;Li KS;Poon RW;Wong BH;Tsoi HW;Yip BC;Huang Y;Chan KH;Yuen KY

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直到最近在中国马蹄蝠中发现了蝙蝠- sars冠状病毒,在其他蝙蝠物种中发现了一种新的1族冠状病毒,人们才知道蝙蝠中是否存在冠状病毒。在16个月的时间里,在香港农村地区的20个不同地点捕获了13种309只蝙蝠,通过RT-PCR从37只蝙蝠(12%)的肛门拭子中扩增出冠状病毒。rna依赖性rna聚合酶(pol)和解旋酶基因的系统发育分析显示,除了先前描述的两种冠状病毒外,还发现了来自六种不同蝙蝠物种的六种新型冠状病毒。在这6种新型冠状病毒中,1组冠状病毒有4种(来自中华马蹄蝠的HKU2型、来自李氏大脚蝠的HKU6型、来自大弯翅蝠的HKU7型和来自小弯翅蝠的HKU8型),2组冠状病毒有2种(来自小竹蝠的HKU4型和来自日本尖鼻蝇的HKU5型)。在蝙蝠身上观察到惊人的冠状病毒多样性。
The existence of coronaviruses in bats is unknown until the recent discovery of bat-SARS-CoV in Chinese horseshoe bats and a novel group 1 coronavirus in other bat species. Among 309 bats of 13 species captured from 20 different locations in rural areas of Hong Kong over a 16-month period, coronaviruses were amplified from anal swabs of 37 (12%) bats by RT-PCR. Phylogenetic analysis of RNA-dependent-RNA-polymerase (pol) and helicase genes revealed six novel coronaviruses from six different bat species, in addition to the two previously described coronaviruses. Among the six novel coronaviruses, four were group 1 coronaviruses (bat-CoV HKU2 from Chinese horseshoe bat, bat-CoV HKU6 from rickett's big-footed bat, bat-CoV HKU7 from greater bent-winged bat and bat-CoV HKU8 from lesser bent-winged bat) and two were group 2 coronaviruses (bat-CoV HKU4 from lesser bamboo bats and bat-CoV HKU5 from Japanese pipistrelles). An astonishing diversity of coronaviruses was observed in bats.
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发表时间: 2004-05-01
影响因子: 9.4
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发表时间: 2005-01-01
影响因子: 2.6
作者:
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发表时间: 2004-04-20
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