Structural and functional features of the 37-kDa 2-5A-dependent RNase L in chronic fatigue syndrome.
Structural and functional features of the 37-kDa 2-5A-dependent RNase L in chronic fatigue syndrome.
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慢性疲劳综合征中 37-kDa 2-5A 依赖性 RNase L 的结构和功能特征。
DOI：
10.1089/10799900252952235
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Suhadolnik,
中科院分区：
文献类型：
--
作者：
Shetzline,SusanE;Martinand-Mari,Camille;Reichenbach,NancyL;Buletic,Zivjena;Lebleu,Bernard;Pfleiderer,Wolfgang;Charubala,Ramamurthy;DeMeirleir,Kenny;DeBecker,Pascale;Peterson,DanielL;Herst,CVT;Englebienne,Patrick;Suhadolnik,
在慢性疲劳综合征（CFS）患者的外周血单个核细胞（PBMC）提取物中发现了一种2 ',5 ' -寡腺苷酸（2- 5a）依赖的37-kDa形式的RNase L。本研究采用分析凝胶渗透FPLC、叠氮光亲和标记、二维凝胶电泳和基质辅助激光解吸/电离质谱（MALDI-MS）等方法，研究了CFS患者PBMC中80 kda RNase L与37 kda RNase L之间的生化关系。与80 kda的RNase L一样，37 kda的RNase L与RNase L抑制剂（RLI）以催化无活性异二聚体复合物的形式存在。37-kDa RNase L- rli复合物的形成表明37-kDa RNase L在n端结构上与80-kDa RNase L相似，含有2-5A结合域。37-kDa RNase L的酶活性单体通过二维凝胶电泳分离得到pI为6.1。RT-PCR和Southern blot分析表明，37 kda的RNase L不是通过选择性剪接形成的。从2-D凝胶中切除的37-kDa RNase L的凝胶内胰蛋白酶消化和随后的MALDI-MS分析鉴定出三个肽团与80-kDa RNase L中的三个预测肽团相同。2-5A叠氮光标记/免疫沉淀的37-kDa RNase L的电泳迁移率在还原和非还原条件下相同。结果表明，PBMC中37-kDa形式的RNase L与天然80-kDa形式的RNase L具有相同的结构和功能特征，特别是在2-5A结合和催化结构域。
A 2′,5′-oligoadenylate (2-5A)-dependent 37-kDa form of RNase L has been reported in extracts of peripheral blood mononuclear cells (PBMC) from individuals with chronic fatigue syndrome (CFS). In the current study, analytic gel permeation FPLC, azido photoaffinity labeling, two-dimensional (2-D) gel electrophoresis, and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) have been used to examine the biochemical relationship between the 80-kDa RNase L in healthy control PBMC and the 37-kDa RNase L in PBMC from individuals with CFS. Like the 80-kDa RNase L, the 37-kDa RNase L is present as a catalytically inactive heterodimer complex with the RNase L inhibitor (RLI). Formation of a 37-kDa RNase L-RLI complex indicates that the 37-kDa RNase L is structurally similar to the 80-kDa RNase L at the N-terminus, which contains the 2-5A binding domain. The enzymatically active monomer form of 37-kDa RNase L resolved by 2-D gel electrophoresis has a pI of 6.1. RT-PCR and Southern blot analyses demonstrated that the 37-kDa RNase L is not formed by alternative splicing. In-gel tryptic digestion of the 37-kDa RNase L that was excised from 2-D gels and subsequent MALDI-MS analysis identified three peptide masses that are identical to three predicted peptide masses in the 80-kDa RNase L. The electrophoretic mobility of 2-5A azido photolabeled/immunoprecipitated 37-kDa RNase L was the same under reducing and nonreducing conditions. The results presented show that the 37-kDa form of RNase L in PBMC shares structural and functional features with the native 80-kDa RNase L, in particular in the 2-5A binding and catalytic domains.
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DOI：
--
发表时间：
1987
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
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2′,5′-寡腺苷酸 (2–5A) 依赖性 37-kDa RNase L 的表征
影响因子：
4.8
作者：
Susan E. Shetzline;R. Suhadolnik
通讯作者：
R. Suhadolnik
使用聚 (I)-聚 (C12U) 治疗慢性疲劳综合征的对照临床试验中 2-5A 合成酶/RNase L 抗病毒途径的变化。
DOI：
--
发表时间：
1994
期刊：
In vivo (Athens, Greece)
影响因子：
--
作者：
Suhadolnik,RJ;Reichenbach,NL;Hitzges,P;Adelson,ME;Peterson,DL;Cheney,P;Salvato,P;Thompson,C;Loveless,M;Müller,WE
通讯作者：
Müller,WE
影响因子：
--
作者：
Jason, LA;Richman, JA;Plioplys, S
通讯作者：
Plioplys, S
慢性疲劳综合征的分子基础的初步确定。
DOI：
--
发表时间：
1996
期刊：
Biochemical and Molecular Medicine
影响因子：
--
作者：
N. McGregor;R. Dunstan;M. Zerbes;H. Butt;T. Roberts;I. Klineberg
通讯作者：
I. Klineberg