Focus on molecules: transglutaminase 2.
Focus on molecules: transglutaminase 2.
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DOI：
10.1016/j.exer.2010.06.006
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发表时间：
2011-07
影响因子：
3.4
通讯作者：
Wordinger, Robert J.
中科院分区：
文献类型：
--
作者：
Tovar-Vidales, Tara;Clark, Abbot F.;Wordinger, Robert J.
转氨酶2，也称为TGM2、tTG、TG2、TGC和G-α-h（登录号：核苷酸NM_004613. 2，蛋白质NP_004604。2; EC 2.3. 2.13）由于选择性剪接而具有两种异构体：（a）具有13个外显子，编码687个氨基酸（77kDa）;（B）具有10个外显子，编码548个氨基酸（61.7kDa）。人类转氨酶2基因（TGM2）位于染色体20q12上。转氨酶2通过未知的非常规机制分泌，因为它缺乏信号序列。尽管存在潜在的糖基化位点，但尚未报告糖基化的证据。X射线晶体学显示，TGM2有四个结构域：一个N端β-夹心结构域（1 - 139），一个转酰胺核心结构域（140 - 454）和两个C端β-桶（分别为479 - 585和586 - 687）（图1）。所有四个领域都有特定的作用。例如，N-末端结构域与纤连蛋白相互作用（Collighan RJ和Griffin M，2009）。转酰胺核心结构域参与GTP结合（Ser171和Lys173），具有活性三联体位点（Cys277、His335和Asp358）和钙结合区。C-末端结构域调节转酰胺活性、GT酶活性，以及隐藏活性位点使其不与可能的底物接触。在钙结合后，活性位点和C-末端结构域之间的相互作用被释放，允许转酰胺酶活性。
Transglutaminase 2, also known as TGM2, tTG, TG2, TGC, and G-alpha-h (Accession numbers: Nucleotide NM_004613. 2, Protein NP_004604. 2; EC 2.3. 2.13) has two isoforms due to alternative splicing:(a) has 13 exons encoding 687 amino acids (77kDa) and (b) has 10 exons encoding 548 amino acids (61.7 kDA). The human transglutaminase 2 gene (TGM2) is located on chromosome 20q12. Transglutaminase 2 is secreted by an unknown non-conventional mechanism, since it lacks a signal sequence. Although potential glycosylation sites are present, no evidence of glycosylation has been reported. X-ray crystallography has revealed that TGM2 has four domains: an N-terminal β-sandwich domain (1-139), a transamidation core domain (140-454), and two C-terminal β-barrels (479-585 and 586-687, respectively)(Figure1). All four domains have particular roles. For example, the N-terminal domain interacts with fibronectin (Collighan RJ and Griffin M, 2009). The transamidation core domain is involved in GTP binding (Ser171 and Lys173), has an active triad site (Cys277, His335, and Asp358), and a calcium binding region. The C-terminal domain regulates transamidation activity, GTPase activity, as well as concealing the active site from contact with possible substrates. Upon calcium binding, the interaction between the active site and C-terminal domains is released, allowing transamidation enzyme activity.
影响因子：
4.4
作者：
Tovar-Vidales, Tara;Roque, Rouel;Wordinger, Robert J.
通讯作者：
Wordinger, Robert J.
影响因子：
3.5
作者：
Collighan, R. J.;Griffin, M.
通讯作者：
Griffin, M.
影响因子：
4.1
作者：
Wan, XH;Lee, EH;Lee, JH
通讯作者：
Lee, JH