Differential human gut microbiome assemblages during soil-transmitted helminth infections in Indonesia and Liberia.
Differential human gut microbiome assemblages during soil-transmitted helminth infections in Indonesia and Liberia.
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DOI：
10.1186/s40168-018-0416-5
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发表时间：
2018-02-28
期刊：
影响因子：
15.5
通讯作者：
Mitreva M
中科院分区：
文献类型：
--
作者：
Rosa BA;Supali T;Gankpala L;Djuardi Y;Sartono E;Zhou Y;Fischer K;Martin J;Tyagi R;Bolay FK;Fischer PU;Yazdanbakhsh M;Mitreva M
人类肠道及其微生物群是土源性蠕虫 (STH) 最常见的感染部位，影响着全球约 15 亿人的福祉。复杂的跨王国相互作用尚不清楚。横断面分析确定了利比里亚和印度尼西亚与 STH 感染呈正相关或负相关的保守微生物特征，双盲随机试验的纵向样本分析表明，肠道微生物群对驱虫有反应，但不会转变为更接近未感染状态。与仍然感染的个体相比，能够自我清除感染的个体的微生物组具有更相似的微生物组组合。一种细菌分类群（毛螺菌科）与两国的感染呈负相关，12 种细菌分类群与两国的 STH 感染显着相关，其中包括奥尔森氏菌（与减少肠道炎症相关），其数量在感染清除后也显着减少。微生物群落基因丰度也受到驱虫的影响。与 STH 感染相关的功能类别包括花生四烯酸代谢；花生四烯酸是威胁蠕虫生存的促炎性白三烯的前体，我们的研究结果表明，STH 感染的肠道中花生四烯酸活性的某些调节可能是通过花生四烯酸代谢细菌的增加而发生的。我们首次使用两种不同的统计方法确定了肠道微生物组的特定成员，这些成员可以区分不同地理区域的中度/重度 STH 感染和未感染状态。我们还确定了与 STH 感染相关的微生物组编码的生物功能，这些功能可能与 STH 生存策略和宿主环境的变化相关。这些结果通过在分类学、遗传和功能水平上解锁微生物组组合，为人类肠道生态系统中跨界相互作用提供了新的见解，从而可以在关键机制研究方面取得进展。本文的在线版本 (10.1186/s40168-018-0416-5) 包含补充材料，可供授权用户使用。
The human intestine and its microbiota is the most common infection site for soil-transmitted helminths (STHs), which affect the well-being of ~ 1.5 billion people worldwide. The complex cross-kingdom interactions are not well understood. A cross-sectional analysis identified conserved microbial signatures positively or negatively associated with STH infections across Liberia and Indonesia, and longitudinal samples analysis from a double-blind randomized trial showed that the gut microbiota responds to deworming but does not transition closer to the uninfected state. The microbiomes of individuals able to self-clear the infection had more alike microbiome assemblages compared to individuals who remained infected. One bacterial taxon (Lachnospiracae) was negatively associated with infection in both countries, and 12 bacterial taxa were significantly associated with STH infection in both countries, including Olsenella (associated with reduced gut inflammation), which also significantly reduced in abundance following clearance of infection. Microbial community gene abundances were also affected by deworming. Functional categories identified as associated with STH infection included arachidonic acid metabolism; arachidonic acid is the precursor for pro-inflammatory leukotrienes that threaten helminth survival, and our findings suggest that some modulation of arachidonic acid activity in the STH-infected gut may occur through the increase of arachidonic acid metabolizing bacteria. For the first time, we identify specific members of the gut microbiome that discriminate between moderately/heavily STH-infected and non-infected states across very diverse geographical regions using two different statistical methods. We also identify microbiome-encoded biological functions associated with the STH infections, which are associated potentially with STH survival strategies, and changes in the host environment. These results provide a novel insight of the cross-kingdom interactions in the human gut ecosystem by unlocking the microbiome assemblages at taxonomic, genetic, and functional levels so that advances towards key mechanistic studies can be made. The online version of this article (10.1186/s40168-018-0416-5) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Kanehisa M;Goto S;Sato Y;Furumichi M;Tanabe M
通讯作者：
Tanabe M
蠕虫感染和肠道微生物群 - 猫的视角。
影响因子：
3.2
作者：
Duarte AM;Jenkins TP;Latrofa MS;Giannelli A;Papadopoulos E;de Carvalho LM;Nolan MJ;Otranto D;Cantacessi C
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影响因子：
6.7
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Broadhurst MJ;Ardeshir A;Kanwar B;Mirpuri J;Gundra UM;Leung JM;Wiens KE;Vujkovic-Cvijin I;Kim CC;Yarovinsky F;Lerche NW;McCune JM;Loke P
通讯作者：
Loke P
影响因子：
5.6
作者：
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通讯作者：
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影响因子：
2.4
作者：
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通讯作者：
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