Helminth infections and gut microbiota - a feline perspective.
Helminth infections and gut microbiota - a feline perspective.
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蠕虫感染和肠道微生物群 - 猫的视角。
DOI：
10.1186/s13071-016-1908-4
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发表时间：
2016-12-03
影响因子：
3.2
通讯作者：
Cantacessi C
中科院分区：
文献类型：
--
作者：
Duarte AM;Jenkins TP;Latrofa MS;Giannelli A;Papadopoulos E;de Carvalho LM;Nolan MJ;Otranto D;Cantacessi C
由于需要更好地了解与寄生虫相关的肠道菌群组成变化对宿主营养不良和免疫调节的影响，科学界对肠道微生物群和胃肠道寄生线虫之间关系的研究越来越感兴趣。然而，这些研究主要是在人类和实验动物中进行的，而对家畜中存在或不存在寄生线虫感染时肠道共生菌群的组成的了解是有限的。在这项研究中，我们研究了广泛存在的猫寄生虫（即猫弓蛔虫）感染对猫宿主肠道微生物群的定性和定量影响。通过对细菌 16S rRNA 基因的 V3-V4 高变区进行高通量测序，然后对序列数据进行生物信息学和生物统计分析，检查了明显感染 T. cati (= Tc+) 以及阴性对照 (= Tc-) 的猫的粪便微生物群。本研究分析的粪便样本总共生成了 2,325,366 个可用的高质量序列，并进行了进一步的生物信息学分析，分别鉴定了 128 个 OTU 和 9 个细菌门。在所有分析的样品中，厚壁菌门占主导地位（平均值为 53.0%），其次是变形菌门（13.8%）、放线菌门（13.7%）和拟杆菌门（10.1%）。其中，与Tc-样品相比，乳杆菌目、肠球菌科、肠球菌属和Dorea属的细菌在Tc+ 中表现出丰度增加的趋势，而Tc+ 和Tc-样品之间的OTU丰富度和多样性没有显着差异（分别为P = 0.485和P = 0.581）。然而，典型相关性和冗余分析能够根据感染状态分离样本（分别为 P = 0.030 和 P = 0.015），这表明后者与猫科动物粪便微生物群的组成之间存在相关性。尽管分析的样本数量相对较少，但可以识别出 Tc+ 与 Tc- 猫肠道微生物群组成的细微差异，其中一些差异与人类和实验动物宿主的当前数据一致。尽管如此，这项研究的结果为家畜寄生虫与微生物相互作用这一尚未被探索的领域提供了宝贵的知识。本文的在线版本 (doi:10.1186/s13071-016-1908-4) 包含补充材料，可供授权用户使用。
Investigations of the relationships between the gut microbiota and gastrointestinal parasitic nematodes are attracting growing interest by the scientific community, driven by the need to better understand the contribution of parasite-associated changes in the composition of the gut flora to both host malnutrition and immune modulation. These studies have however been carried out mainly in humans and experimental animals, while knowledge of the make-up of the gut commensal flora in presence or absence of infection by parasitic nematodes in domestic animals is limited. In this study, we investigate the qualitative and quantitative impact that infections by a widespread parasite of cats (i.e. Toxocara cati) exert on the gut microbiota of feline hosts. The faecal microbiota of cats with patent infection by T. cati (= Tc+), as well as that of negative controls (= Tc-) was examined via high-throughput sequencing of the V3-V4 hypervariable region of the bacterial 16S rRNA gene, followed by bioinformatics and biostatistical analyses of sequence data. A total of 2,325,366 useable high-quality sequences were generated from the faecal samples analysed in this study and subjected to further bioinformatics analyses, which led to the identification of 128 OTUs and nine bacterial phyla, respectively. The phylum Firmicutes was predominant in all samples analysed (mean of 53.0%), followed by the phyla Proteobacteria (13.8%), Actinobacteria (13.7%) and Bacteroidetes (10.1%). Among others, bacteria of the order Lactobacillales, the family Enterococcaceae and genera Enterococcus and Dorea showed a trend towards increased abundance in Tc+ compared with Tc- samples, while no significant differences in OTU richness and diversity were recorded between Tc+ and Tc- samples (P = 0.485 and P = 0.581, respectively). However, Canonical Correlation and Redundancy Analyses were able to separate samples by infection status (P = 0.030 and P = 0.015, respectively), which suggests a correlation between the latter and the composition of the feline faecal microbiota. In spite of the relatively small number of samples analysed, subtle differences in the composition of the gut microbiota of Tc+ vs Tc- cats could be identified, some of which in accordance with current data from humans and laboratory animal hosts. Nevertheless, the findings from this study contribute valuable knowledge to the yet little explored area of parasite-microbiota interactions in domestic animals. The online version of this article (doi:10.1186/s13071-016-1908-4) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Klindworth A;Pruesse E;Schweer T;Peplies J;Quast C;Horn M;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
4.6
作者：
Li RW;Li W;Sun J;Yu P;Baldwin RL;Urban JF
通讯作者：
Urban JF
DOI：
10.1098/rstb.2014.0296
发表时间：
2015-08-19
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Giacomin P;Croese J;Krause L;Loukas A;Cantacessi C
通讯作者：
Cantacessi C
影响因子：
3.1
作者：
Li, Robert W.;Wu, Sitao;Urban, Joseph F., Jr.
通讯作者：
Urban, Joseph F., Jr.
DOI：
10.1099/00207713-50-3-979
发表时间：
2000-05-01
影响因子：
2.8
作者：
Downes, J;Olsvik, B;Wade, WG
通讯作者：
Wade, WG