Ontogeny and cellular origin of an interleukin-1-like factor in the reproductive tract of the male rat.
Ontogeny and cellular origin of an interleukin-1-like factor in the reproductive tract of the male rat.
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雄性大鼠生殖道中白细胞介素 1 样因子的个体发育和细胞起源。
DOI：
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发表时间：
1988
影响因子：
--
通讯作者：
E. Ritzén
中科院分区：
文献类型：
--
作者：
V. Syed;O. Söder;S. Arver;M. Lindh;S. Khan;E. Ritzén
我们最近从大鼠睾丸中分离出一种白细胞介素-1 （IL-1）样因子，它起源于精小管，是一种分子量为17000，pI为5-6的蛋白质。本文报道了il -1样因子在出生后发育过程中的出现，并进一步探讨了其细胞起源。用小鼠胸腺细胞增殖法测定IL-1活性。10日龄或20日龄大鼠精管培养培养基中IL-1活性极低。从30日龄开始，检测到的含量逐渐增加，在成年动物（60-90日龄）达到最大值。在小管周围细胞调节的培养基中未发现IL-1活性。从实验隐睾或产前辐照大鼠获得的富含支持细胞的精管产生的IL-1活性水平比从完整睾丸获得的高得多。结扎传出管24 h后，检测传出管液中IL-1活性，提示IL-1样因子已分泌到管腔内。在附睾组织和附睾精子提取物中检测到低水平的IL-1活性，而射精浆、精囊液和精囊提取物（连同凝固腺）以及前列腺腹侧和背外侧缺乏IL-1活性。相反，精浆抑制睾丸IL-1活性的剂量依赖性，而不影响胸腺细胞培养中的细胞活力。尽管其生物学功能仍有待明确，但我们的研究结果表明睾丸il -1样因子是由支持细胞产生的，其在发育过程中的出现与大鼠睾丸中活跃精子发生的开始相吻合。
We have recently isolated an interleukin-1 (IL-1)-like factor from the rat testis, which originates from the seminiferous tubules and is a protein with an MW of 17,000 and a pI of 5-6. This paper reports on the appearance of the IL-1-like factor during postnatal development and investigates its cellular origin further. IL-1 activity was measured by a murine thymocyte proliferation assay. Very low IL-1 activity was present in culture medium conditioned by seminiferous tubules from rats aged 10 or 20 days. From 30 days of age, increasing amounts were detected, reaching a maximum level in adult animals (60-90 days). No IL-1 activity was found in medium conditioned by peritubular cells. Sertoli cell-enriched seminiferous tubules obtained from experimentally cryptorchid or from prenatally irradiated rats produced much higher levels of IL-1 activity than did those obtained from intact testes. IL-1 activity was detected in efferent duct fluid after ligation of the efferent ducts for 24 h, indicating that the IL-1-like factor was secreted into the tubular lumen. Low levels of IL-1 activity were detected in extracts of epididymal tissue and epididymal sperm, whereas ejaculated seminal plasma, seminal vesicle fluid and extracts of seminal vesicles (together with the coagulating glands) and ventral and dorsolateral prostate lacked IL-1 activity. Instead, seminal plasma inhibited testicular IL-1 activity dose-dependently without affecting cell viability in the thymocyte cultures. Although its biological function remains to be defined, our results indicate that the testicular IL-1-like factor is produced by Sertoli cells and that its appearance during development coincides with the initiation of active spermatogenesis in the rat testis.
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大鼠支持细胞分泌一种生长因子，可阻止表皮生长因子 (EGF) 与其受体结合。
DOI：
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发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Holmes,SD;Spotts,G;Smith,RG
通讯作者：
Smith,RG
来自人单核细胞白血病细胞的白介素-1 诱导成纤维细胞增殖。
影响因子：
--
作者：
Postlethwaite,AE;Lachman,LB;Kang,AH
通讯作者：
Kang,AH
来自培养的大鼠支持细胞培养基的生长调节素样肽的部分表征。
影响因子：
4.8
作者：
Smith,EP;Svoboda,ME;VanWyk,JJ;Kierszenbaum,AL;Tres,LL
通讯作者：
Tres,LL
DOI：
10.1073/pnas.77.8.4774
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
FEIG, LA;BELLVE, AR;KLAGSBRUN, M
通讯作者：
KLAGSBRUN, M