Impact of CD68/(CD3+CD20) ratio at the invasive front of primary tumors on distant metastasis development in breast cancer.
Impact of CD68/(CD3+CD20) ratio at the invasive front of primary tumors on distant metastasis development in breast cancer.
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DOI：
10.1371/journal.pone.0052796
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Vizoso FJ
中科院分区：
文献类型：
--
作者：
Eiró N;Pidal I;Fernandez-Garcia B;Junquera S;Lamelas ML;del Casar JM;González LO;López-Muñiz A;Vizoso FJ
肿瘤被巨噬细胞、T 淋巴细胞和 B 淋巴细胞浸润，这可能通过促进血管生成、生长和侵袭来促进肿瘤的发展。本研究的目的是探讨乳腺癌侵袭前沿巨噬细胞 (CD68+)、T 细胞 (CD3+) 和 B 细胞 (CD20+) 相对数量，以及侵袭前沿或肿瘤中心基质金属蛋白酶 (MMP) 及其抑制剂 (TIMP) 表达的临床相关性。我们进行了一项免疫组织化学研究，对 102 例乳腺癌的侵袭前沿的 CD3、CD20 和 CD68 阳性细胞进行了计数。此外，用MMP-2、-9、-11、-14和TIMP-2抗体对组织切片进行染色，并确定侵袭前沿和肿瘤中心的免疫反应位置、反应面积百分比和强度。结果显示，CD68 计数和 CD68/(CD3+CD20) 比值增加与肿瘤中心单核炎症细胞表达 MMP-11 和 TIMP-2 直接相关（CD68 计数 p = 0.041 和 p = 0.025，比率 p = 0.001 和 p = 0.045对于 MMP-11 和 TIMP-2）。此外，高CD68/(CD3+CD20)比率(>0.05)与缩短无复发生存的可能性直接相关。多变量分析显示CD68/(CD3+CD20)比值是与远处无复发生存相关的独立因素(RR: 2.54, CI: (1.23–5.24), p<0.01)。因此，侵袭前沿的CD68/(CD3+CD20)比值可作为重要的预后标志物。
Tumors are infiltrated by macrophages, T and B-lymphocytes, which may favor tumor development by promoting angiogenesis, growth and invasion. The aim of this study was to investigate the clinical relevance of the relative amount of macrophages (CD68+), T-cells (CD3+) and B-cells (CD20+) at the invasive front of breast carcinomas, and the expression of matrix metalloproteases (MMPs) and their inhibitors (TIMPs) either at the invasive front or at the tumor center. We performed an immunohistochemical study counting CD3, CD20 and CD68 positive cells at the invasive front, in 102 breast carcinomas. Also, tissue sections were stained with MMP-2, -9, -11, -14 and TIMP-2 antibodies, and immunoreactivity location, percentage of reactive area and intensity were determined at the invasive front and at the tumor center. The results showed that an increased CD68 count and CD68/(CD3+CD20) ratio were directly associated with both MMP-11 and TIMP-2 expression by mononuclear inflammatory cells at the tumor center (p = 0.041 and p = 0.025 for CD68 count and p = 0.001 and p = 0.045 for ratio, respectively for MMP-11 and TIMP-2). In addition, a high CD68/(CD3+CD20) ratio (>0.05) was directly associated with a higher probability of shortened relapse-free survival. Multivariate analysis revealed that CD68/(CD3+CD20) ratio was an independent factor associated with distant relapse-free survival (RR: 2.54, CI: (1.23–5.24), p<0.01). Therefore, CD68/(CD3+CD20) ratio at the invasive front could be used as an important prognostic marker.
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影响因子：
50.3
作者：
Hanahan, Douglas;Coussens, Lisa M.
通讯作者：
Coussens, Lisa M.
DOI：
10.4049/jimmunol.0903009
发表时间：
2010-04-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
DiLillo DJ;Yanaba K;Tedder TF
通讯作者：
Tedder TF
影响因子：
20.3
作者：
Coughlin, CM;Vance, BA;Vonderheide, RH
通讯作者：
Vonderheide, RH
影响因子：
5.1
作者：
Griffiths, L;Binley, K;Naylor, S
通讯作者：
Naylor, S
影响因子：
8.8
作者：
Gonzalez, L. O.;Pidal, I.;Junquera, S.;Corte, M. D.;Vazquez, J.;Rodriguez, J. C.;Lamelas, M. L.;Merino, A. M.;Garcia-Muniz, J. L.;Vizoso, F. J.
通讯作者：
Vizoso, F. J.