Fluorescence polarization/anisotropy in diagnostics and imaging.
Fluorescence polarization/anisotropy in diagnostics and imaging.
复制标题
DOI：
10.1021/cr900267p
复制
发表时间：
2010-05-12
期刊：
影响因子：
62.1
通讯作者：
Ross, Justin A.
中科院分区：
文献类型：
--
作者：
Jameson, David M.;Ross, Justin A.
近60年前，荧光偏振首次应用于生物化学，当时Gregorio Weber描述了他对牛血清白蛋白和卵白蛋白与1-二甲氨基萘-5-磺酰氯偶联的研究1,2（图1）。（关于Gregorio Weber对荧光的广泛贡献的概述，请参见参考文献3）。在韦伯的研究之后的几十年里，极化方法变得越来越流行。然而，在过去的几十年里，荧光偏振研究的数量和多样性的增加是惊人的，该方法现在在临床和生物医学领域非常广泛。极化研究的爆发始于20世纪80年代中期，这是由于几个因素，包括配备偏振器的商用仪器的可用性，大量荧光探针的商用可用性（主要归功于分子Probessnow公司Invitrogen的一部分），以及在临床化学领域，雅培公司引入了TDx仪器（及相关试剂）。荧光偏振在临床和高通量检测中流行的原因是多方面的。首先，极化分析是均匀的；也就是说，没有必要分离自由配体和结合配体（这些类型的测定通常被称为“混合测量”测定）。其次，开发基于荧光的检测方法的最初动机之一是不需要放射性同位素。第三，极化分析是可重复的，并且易于自动化。在这篇评论中，我们将简要地追溯该技术的历史，并详细讨论理论和实践方面。我们还将从文献中提供大量的例子，说明该方法在配体结合、免疫测定、高通量筛选和活细胞成像等领域的范围，并暗示未来的方向和应用。
Fluorescence polarization was first applied in biochemistry almost 6 decades ago, when Gregorio Weber described his studies on bovine serum albumin and ovalbumin conjugated with 1-dimethylaminonaphthalene-5-sulfonyl chloride (dansyl chloride) 1, 2 (Figure 1).(For an overview of Gregorio Weber’s wide-ranging contributions to fluorescence, see ref 3). Polarization methods became increasingly popular during the decades following Weber’s work. During the past few decades, however, the increase in the number and diversity of fluorescence polarization studies has been astonishing and the method is now extremely widespread in the clinical and biomedical fields. The virtual explosion of polarization studies, which began during the mid-1980s, was due to several factors, including the availability of commercial instruments equipped with polarizers, the commercial availability of a great many fluorescence probes (largely due to the company Molecular Probessnow part of Invitrogen), and, in the clinical chemistry area, the introduction of the TDx instrument (and associated reagents) by Abbott Laboratories. The reasons for the popularity of fluorescence polarization in clinical and high-throughput assays are manifold. First, polarization assays are homogeneous; that is, there is no necessity for separation of free and bound ligand (these types of assays are often referred to as “mix and measure” assays). Second, and one of the original motivations for the development of fluorescence-based assays, there is no need for radioisotopes. Third, polarization assays are reproducible and facilely automated. In this review we shall briefly trace the history of the technique and discuss in detail both theoretical and practical aspects. We shall also present copious examples from the literature, which illustrate the scope of the method in areas such as ligand binding, immunoassays, highthroughput screening, and live cell imaging and hint at future directions and applications.
登录
查看更多内容
影响因子：
2.8
作者：
ARBELOA, IL;OJEDA, PR
通讯作者：
OJEDA, PR
影响因子：
3.4
作者：
BOS, MA;KLEIJN, JM
通讯作者：
KLEIJN, JM
影响因子：
4.4
作者：
AZUMI, T;MCGLYNN, SP
通讯作者：
MCGLYNN, SP
影响因子：
3.4
作者：
Borejdo, J;Akopova, I
通讯作者：
Akopova, I
影响因子：
3.4
作者：
BOREJDO, J;BURLACU, S
通讯作者：
BURLACU, S