Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction.
Use of Precision-Cut Tissue Slices as a Translational Model to Study Host-Pathogen Interaction.
复制标题
DOI：
10.3389/fvets.2021.686088
复制
发表时间：
2021
影响因子：
3.2
通讯作者：
Werling D
中科院分区：
文献类型：
--
作者：
Majorova D;Atkins E;Martineau H;Vokral I;Oosterhuis D;Olinga P;Wren B;Cuccui J;Werling D
最近，分析宿主-病原体相互作用的新技术的增加促进了开发新方法的竞赛，不仅在细胞水平上，而且在组织水平上评估这些方法。由于小鼠与其他哺乳动物的差异，迫切需要开发相关的组织模型，以更紧密地概括宿主组织在疾病和修复过程中的情况。尽管类器官和器官芯片技术有其优点，但它们仍然不能提供宿主组织的细胞和结构复杂性。在这里，精确切割组织切片（PCTS）可以为复杂的离体组织提供宝贵的模型，以“整个器官”的方式评估宿主-病原体相互作用以及潜在的疫苗反应。在这篇小型综述中，我们讨论了目前关于兽医物种PCTS的文献，并主张PCTS是进一步缩小靶点识别、随后将结果转化为临床研究之间差距的重要工具，从而为未来的精准医学方法开辟了道路。
The recent increase in new technologies to analyze host-pathogen interaction has fostered a race to develop new methodologies to assess these not only on the cellular level, but also on the tissue level. Due to mouse-other mammal differences, there is a desperate need to develop relevant tissue models that can more closely recapitulate the host tissue during disease and repair. Whereas organoids and organs-on-a-chip technologies have their benefits, they still cannot provide the cellular and structural complexity of the host tissue. Here, precision cut tissue slices (PCTS) may provide invaluable models for complex ex-vivo generated tissues to assess host-pathogen interaction as well as potential vaccine responses in a “whole organ” manner. In this mini review, we discuss the current literature regarding PCTS in veterinary species and advocate that PCTS represent remarkable tools to further close the gap between target identification, subsequent translation of results into clinical studies, and thus opening avenues for future precision medicine approaches.
登录
查看更多内容
影响因子：
3.7
作者：
Wu, Wenxin;Booth, J. Leland;Metcalf, Jordan P.
通讯作者：
Metcalf, Jordan P.
影响因子：
4.4
作者：
Kirchhoff, Jana;Uhlenbruck, Sabine;Herrler, Georg
通讯作者：
Herrler, Georg
影响因子：
--
作者：
Belanger，Maura C.;Ball，Alexander G.;Pompano，Rebecca R.
通讯作者：
Pompano，Rebecca R.
影响因子：
3.7
作者：
Neuhaus V;Schwarz K;Klee A;Seehase S;Förster C;Pfennig O;Jonigk D;Fieguth HG;Koch W;Warnecke G;Yusibov V;Sewald K;Braun A
通讯作者：
Braun A
影响因子：
16.6
作者：
Abolins S;King EC;Lazarou L;Weldon L;Hughes L;Drescher P;Raynes JG;Hafalla JCR;Viney ME;Riley EM
通讯作者：
Riley EM