Choosing the right antibody for resistin-like molecule (RELM/FIZZ) family members.
Choosing the right antibody for resistin-like molecule (RELM/FIZZ) family members.
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DOI：
10.1007/s00418-012-1042-0
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发表时间：
2013-04
影响因子：
2.3
通讯作者：
Johns RA
中科院分区：
文献类型：
--
作者：
Fan C;Johns BA;Su Q;Kolosova IA;Johns RA
The family of resistin-like molecules (RELM), also known as found in inflammatory zone (FIZZ), consists of four members in mouse (RELMα/FIZZ1/HIMF, RELMβ/FIZZ2, Resistin/FIZZ3, and RELMγ/FIZZ4) and two members in human (resistin and RELMβ). The importance of these proteins in many aspects of physiology and pathophysiology, especially inflammatory processes, is rapidly evolving in the literature, and many investigators are beginning to work in this field. Most published studies focus on only one isoform, do not evaluate other isoforms that might be present, and have not tested for the specificity of the antibody used. Because RELM isoforms have high sequence and structural similarity and both distinct and overlapping functions, it is important to use a specific antibody to distinguish each isoform in the study. We constructed and established HEK 293 cell lines that constitutively express each isoform. Using these cell lines, we determined the specificity of antibodies (both commercially available and laboratory-made) to each isoform by Western blot and immunofluorescence. Some of the antibodies showed specificity in Western blotting but were not applicable in immunofluorescence. Others showed cross reactivity in Western blot assays. Our results indicate that RELM antibody specificity should be taken into account when using them in research and interpreting data obtained with them.
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影响因子：
11.4
作者：
Holcomb, IN;Kabakoff, RC;Hébert, CC
通讯作者：
Hébert, CC
DOI：
10.1152/ajplung.00147.2007
发表时间：
2007-08-01
影响因子：
4.9
作者：
Mishra, Anil;Wang, Meiqin;Rothenberg, Marc E.
通讯作者：
Rothenberg, Marc E.
DOI：
10.1073/pnas.98.2.502
发表时间：
2001-01-16
影响因子：
11.1
作者：
Steppan, CM;Brown, EJ;Lazar, MA
通讯作者：
Lazar, MA
影响因子：
4.8
作者：
Kim, KH;Lee, K;Sul, HS
通讯作者：
Sul, HS
DOI：
10.1165/rcmb.2008-0271oc
发表时间：
2009-11-01
影响因子：
6.4
作者：
Angelini, Daniel J.;Su, Qingning;Johns, Roger A.
通讯作者：
Johns, Roger A.