Protective efficacy of two new anti-caries DNA vaccines.
Protective efficacy of two new anti-caries DNA vaccines.
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两种新型抗龋齿 DNA 疫苗的保护功效。
DOI：
10.1016/j.vaccine.2009.05.007
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发表时间：
2009-12
期刊：
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
葡萄糖基转移酶（glucosyltransferases，GTF）的催化区是致龋细菌依赖蔗糖粘附牙齿的关键区域。在这项研究中，我们评估了催化区增强抗龋齿DNA疫苗免疫原性的潜力。我们将不同类型的GTF催化区克隆到原质粒pGJA-P/VAX中，构建了两个新的防龋DNA质粒pGJGAC/VAX和pGJGA-5C/VAX。一个是1.1kb的S.另一个是其催化核心序列，其在来自变形链球菌的GTF中是保守的，并且在蔗糖裂解和葡聚糖合成的酶活性中起核心作用。防龋实验结果表明，与pGJA-P/VAX相比，两种新质粒免疫对致龋菌尤其是S. sobrinus。编码全长催化区的质粒比编码催化核心保守序列的质粒对致龋菌的保护作用更强，尽管差异不是很显著。
As we know, the catalytic region of glucosyltransferases (GTFs) is a key region responsible for sucrose-dependent adherence of cariogenic bacteria to teeth. In this study, we evaluate the potential of the catalytic region to enhance the immunogenicity of the anti-caries DNA vaccine. We construct two new anti-caries DNA plasmids pGJGAC/VAX and pGJGA-5C/VAX by cloning different styles of the catalytic regions of GTFs into the previous plasmid pGJA-P/VAX. One is the 1.1kb full length catalytic region of S. sobrinus GTF-I, the other is its catalytic core sequence which is conserved in the GTFs from mutans streptococci and plays a central role in the enzymatic activities of sucrose splitting and glucan synthesis. The results of caries protection experiment indicate that compared to pGJA-P/VAX, immunization with both new plasmids provides more effective protection against cariogenic bacteria, especially against S. sobrinus. The plasmid encoding full length catalytic region could provide more effective protection against cariogenic bacteria than that encoding catalytic core conserved sequence even though the differences are not very dramatic.
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与变形链球菌葡萄糖基转移酶催化结构域相关的合成肽的免疫学特征。
影响因子：
3.1
作者：
Smith,DJ;Taubman,MA;King,WF;Eida,S;Powell,JR;Eastcott,J
通讯作者：
Eastcott,J
影响因子：
7.6
作者：
R. Russell;T. Shiroza;H. Kuramitsu;J. Ferreti
通讯作者：
R. Russell;T. Shiroza;H. Kuramitsu;J. Ferreti
DOI：
10.1016/s0021-9258(18)31531-x
发表时间：
1991-05
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Mooser;S. Hefta;R. Paxton;J. Shively;Terry D. Lee
通讯作者：
G. Mooser;S. Hefta;R. Paxton;J. Shively;Terry D. Lee
影响因子：
4.2
作者：
Qing-an Xu;F. Yu;M. Fan;Z. Bian;Jihua Guo;R. Jia;Zhi Chen;B. Peng;B. Fan
通讯作者：
Qing-an Xu;F. Yu;M. Fan;Z. Bian;Jihua Guo;R. Jia;Zhi Chen;B. Peng;B. Fan
影响因子：
3
作者：
Okada, M;Soda, Y;Kozai, K
通讯作者：
Kozai, K