Genome-Wide Identification of Petunia HSF Genes and Potential Function of PhHSF19 in Benzenoid/Phenylpropanoid Biosynthesis.
Genome-Wide Identification of Petunia HSF Genes and Potential Function of PhHSF19 in Benzenoid/Phenylpropanoid Biosynthesis.
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DOI：
10.3390/ijms23062974
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发表时间：
2022-03-10
影响因子：
5.6
通讯作者：
Zhang C
中科院分区：
文献类型：
--
作者：
Fu J;Huang S;Qian J;Qing H;Wan Z;Cheng H;Zhang C
挥发性苯并/苯丙素类化合物是矮牵牛（Petuniahybrida）的主要花香成分。热休克因子（Heat shock factors，HSFs）是热应激反应的主要调节因子，参与苯类/苯丙素类等次生代谢产物的生物合成。为了明确HSFs在调控矮牵牛花香味中的潜在功能，我们系统鉴定了矮牵牛全基因组HSF基因，分析了其表达情况，并对矮牵牛关键HSF基因与苯/苯丙素类化合物生物合成靶基因之间的相互作用进行了研究。结果表明，在矮牵牛中共获得34个HSF基因家族成员，且大多数矮牵牛HSF基因含有一个内含子。系统发育分析表明，23个矮牵牛HSF聚为最大的亚家族HSFA，而只有2个矮牵牛HSF属于HSFC亚家族。DBD结构域和NLS基序在大多数矮牵牛HSF中是很保守的。大多数矮牵牛HSF基因的启动子含有STRE基序，这是顺式作用元件数量最多的。PhHSF 19在花瓣管中高度表达，其次是花梗和花瓣肢。在花发育过程中，PhHSF 19的表达水平在花开放早期显著高于花蕾期，提示PhHSF 19可能在调控苯类/苯丙素类生物合成中具有潜在作用。PhHSF 19的表达模式与PhPAL 2正相关，PhPAL 2催化苯丙烷途径中的第一个关键步骤。此外，在PhPAL 2的启动子中有三个STRE元件。根据酵母单杂交和双荧光素酶测定，证明PhHSF 19正调控PhPAL 2的表达。这些结果为进一步研究HSFs对植物花香合成的调控奠定了理论基础。
Volatile benzenoids/phenylpropanoids are the main flower scent compounds in petunia (Petunia hybrida). Heat shock factors (HSFs), well known as the main regulator of heat stress response, have been found to be involved in the biosynthesis of benzenoid/phenylpropanoid and other secondary metabolites. In order to figure out the potential function of HSFs in the regulation of floral scent in petunia, we systematically identified the genome-wide petunia HSF genes and analyzed their expression and then the interaction between the key petunia HSF gene with target gene involved in benzenoid/phenylpropanoid biosynthesis. The results revealed that 34 HSF gene family members were obtained in petunia, and most petunia HSFs contained one intron. The phylogenetic analysis showed that 23 petunia HSFs were grouped into the largest subfamily HSFA, while only two petunia HSFs were in HSFC subfamily. The DBD domain and NLS motif were well conserved in most petunia HSFs. Most petunia HSF genes’ promoters contained STRE motifs, the highest number of cis-acting element. PhHSF19 is highly expressed in petal tubes, followed by peduncles and petal limbs. During flower development, the expression level of PhHSF19 was dramatically higher at earlier flower opening stages than that at the bud stage, suggesting that PhHSF19 may have potential roles in regulating benzenoid/phenylpropanoid biosynthesis. The expression pattern of PhHSF19 is positively related with PhPAL2, which catalyzes the first committed step in the phenylpropanoid pathway. In addition, there are three STRE elements in the promoter of PhPAL2. PhHSF19 was proven to positively regulate the expression of PhPAL2 according to the yeast one hybrid and dual luciferase assays. These results lay a theoretical foundation for further studies of the regulation of HSFs on plant flower scent biosynthesis.
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