Division of Labor during Biofilm Matrix Production.
Division of Labor during Biofilm Matrix Production.
复制标题
DOI：
10.1016/j.cub.2018.04.046
复制
发表时间：
2018-06-18
期刊：
影响因子：
--
通讯作者：
Kovács ÁT
中科院分区：
文献类型：
--
作者：
Dragoš A;Kiesewalter H;Martin M;Hsu CY;Hartmann R;Wechsler T;Eriksen C;Brix S;Drescher K;Stanley-Wall N;Kümmerli R;Kovács ÁT
像细菌这样简单的生物体可以参与复杂的集体行动，例如群体运动和子实体形成。这些行动中的一些涉及劳动分工，其中表型专门化的克隆亚群，或遗传上不同的谱系通过执行互补任务相互合作。在这里，我们结合联合收割机的实验和计算方法来研究生物膜基质生产过程中的劳动分工所带来的潜在利益。我们表明，劳动分工的表型和遗传策略可以促进土壤细菌枯草芽孢杆菌集体生物膜的形成。在该物种中，生物膜基质由两种主要组分组成; EPS和TasA。观察到B.枯草芽孢杆菌表型分离成三个亚群组成的矩阵非生产者，EPS生产者，和generalists，产生EPS和TasA。这种不完全的表型特化被遗传分工所超越，在遗传分工中，两个突变体被设计成专家，通过交换EPS和TasA相互补充。这两个突变体的相对适合度显示出负频率依赖性在体外和植物根，与应变频率达到稳定的平衡，在30%的TasA-生产者，对应的人口组成，其中组生产力最大化。使用基于个人的建模，我们表明，在应变比的不对称性可能会出现由于基质化合物产生的集体的相对利益的差异;和遗传分工可以有利于当它打破与同时生产的两个矩阵组件的代谢约束。
Organisms as simple as bacteria can engage in complex collective actions, such as group motility and fruiting body formation. Some of these actions involve a division of labor, where phenotypically specialized clonal subpopulations, or genetically distinct lineages cooperate with each other by performing complementary tasks. Here, we combine experimental and computational approaches to investigate potential benefits arising from division of labor during biofilm matrix production. We show that both phenotypic and genetic strategies for a division of labor can promote collective biofilm formation in the soil bacterium Bacillus subtilis. In this species, biofilm matrix consists of two major components; EPS and TasA. We observed that clonal groups of B. subtilis phenotypically segregate into three subpopulations composed of matrix non-producers, EPS-producers, and generalists, which produce both EPS and TasA. This incomplete phenotypic specialization was outperformed by a genetic division of labor, where two mutants, engineered as specialists, complemented each other by exchanging EPS and TasA. The relative fitness of the two mutants displayed a negative frequency dependence both in vitro and on plant roots, with strain frequency reaching a stable equilibrium at 30% TasA-producers, corresponding exactly to the population composition where group productivity is maximized. Using individual-based modelling, we show that asymmetries in strain ratio can arise due to differences in the relative benefits that matrix compounds generate for the collective; and that genetic division of labor can be favored when it breaks metabolic constraints associated with the simultaneous production of two matrix components.
登录
查看更多内容
影响因子：
5.6
作者：
Hölscher T;Bartels B;Lin YC;Gallegos-Monterrosa R;Price-Whelan A;Kolter R;Dietrich LEP;Kovács ÁT
通讯作者：
Kovács ÁT
影响因子：
6.7
作者：
Alteri CJ;Himpsl SD;Pickens SR;Lindner JR;Zora JS;Miller JE;Arno PD;Straight SW;Mobley HL
通讯作者：
Mobley HL
DOI：
10.3791/54752
发表时间：
2016-10-29
期刊：
Journal of visualized experiments : JoVE
影响因子：
--
作者：
Hölscher T;Dragoš A;Gallegos-Monterrosa R;Martin M;Mhatre E;Richter A;Kovács ÁT
通讯作者：
Kovács ÁT
影响因子：
4.3
作者：
Germerodt S;Bohl K;Lück A;Pande S;Schröter A;Kaleta C;Schuster S;Kost C
通讯作者：
Kost C
影响因子：
3.6
作者：
Kobayashi, Kazuo
通讯作者：
Kobayashi, Kazuo