Dysregulated expression of CD66a (BGP, C-CAM), an adhesion molecule of the CEA family, in endometrial cancer.
Dysregulated expression of CD66a (BGP, C-CAM), an adhesion molecule of the CEA family, in endometrial cancer.
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子宫内膜癌中 CD66a（BGP、C-CAM）（CEA 家族的一种粘附分子）的表达失调。
DOI：
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发表时间：
1998
影响因子：
6
通讯作者：
Thomas Löning
中科院分区：
文献类型：
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作者：
A. Bamberger;L. Riethdorf;P. Nollau;M. Naumann;I. Erdmann;J. Götze;J. Brümmer;Heinrich M. Schulte;Christoph Wagener;Thomas Löning
CD 66 a（BGP，C-CAM）是癌胚抗原家族的粘附分子，其已显示在结直肠癌、前列腺癌和乳腺癌中下调。本研究的目的是确定其在正常人子宫内膜和子宫内膜肿瘤中的表达模式。为此，我们进行了免疫组化使用4D 1/C2单克隆抗体对一系列的24个正常子宫内膜标本和47个子宫内膜癌。在正常子宫内膜的腺上皮细胞和腔上皮细胞中观察到强烈的CD 66 a表达，在整个周期中，这些细胞的顶极处具有一致的定位。在分泌晚期（经前期），细胞极性的丧失导致一些腺细胞的膜表达模式。在分析的肿瘤样品中，随着恶性程度的增加，观察到表达完全丧失的区域增加。在55%的肿瘤中，正常上皮的顶端表达模式改变为膜性全方位模式，大多数在实性区域。这种变化与恶性程度相关，可在15例G1肿瘤中观察到3例，12例G2肿瘤中观察到4例，12例G3肿瘤中观察到11例，8例浆液性乳头状癌中观察到8例。具有膜表达模式的区域可以与具有正常顶端表达模式的区域沿着在低级别癌中观察到，并且与具有完全丧失表达的区域一起在高级别肿瘤中观察到。北方印迹分析显示肿瘤样品和HEC-1B子宫内膜腺癌细胞中mRNA表达缺失。通过Western印迹也观察到肿瘤样品中蛋白质表达的丧失。总之，CD 66 a蛋白表达失调的子宫内膜癌，表现出减少或损失的表达与恶性程度的增加和变化，从顶端到膜定位。
CD66a (BGP, C-CAM) is an adhesion molecule of the carcinoembryonic antigen family that has been shown to be down-regulated in colorectal, prostate, and breast cancers. The purpose of the present study was to determine its expression pattern in the normal human endometrium and in endometrial neoplasia. For this purpose, we performed immunohistochemistry using the 4D1/C2 monoclonal antibody on a series of 24 normal endometrial samples and 47 endometrial carcinomas. Strong CD66a expression was observed in glandular and luminal epithelial cells of the normal endometrium with a consistent localization at the apical poles of these cells throughout the cycle. In late secretory (premenstrual) phase, loss of cellular polarity resulted in a membranous expression pattern in some glandular cells. In the analyzed tumor samples increasing areas with a complete loss of expression were observed with increasing malignancy grade. The apical expression pattern of the normal epithelium was changed to a membranous all-around pattern in 55% of the tumors, mostly in solid areas. This change correlated with malignancy grade and could be observed in 3 of 15 G1 tumors, 4 of 12 G2 tumors, 11 of 12 G3 tumors, and 8 of 8 serous-papillary carcinomas. Areas with membranous expression pattern could be observed along with areas with a normal apical expression pattern in lower grade carcinomas and with areas with complete loss of expression in high grade tumors. Northern blot analysis showed a loss of mRNA expression in tumor samples and HEC-1B endometrial adenocarcinoma cells. Loss of protein expression in the tumor samples was also observed by Western blot. In conclusion, CD66a protein expression is dysregulated in endometrial carcinomas, showing reduction or loss of expression with increasing malignancy grade and a change from the apical to a membranous localization.
影响因子：
11.2
作者：
J. Hsieh;W. Luo;Weitao Song;Yang Wang;D. Kleinerman;Nguyen T. Van;S. Lin
通讯作者：
J. Hsieh;W. Luo;Weitao Song;Yang Wang;D. Kleinerman;Nguyen T. Van;S. Lin
影响因子：
56.9
作者：
Zhang, L;Zhou, W;Kinzler, KW
通讯作者：
Kinzler, KW