Membrane fluidity of halophilic ectoine-secreting bacteria related to osmotic and thermal treatment
Membrane fluidity of halophilic ectoine-secreting bacteria related to osmotic and thermal treatment
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与渗透和热处理相关的嗜盐四氢嘧啶分泌细菌的膜流动性
DOI：
10.1007/s00449-013-0957-8
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发表时间：
2013
影响因子：
3.8
通讯作者：
Krull R.
中科院分区：
文献类型：
--
作者：
Bergmann S;David F;Clark W;Wittmann C;Krull R.
为了应对渗透压的突然下降，嗜盐微生物分泌它们积累的渗透压调节剂。这种特定的应激反应，结合对改变的环境的生理化学反应，影响细胞的膜特性和完整性，从而影响生物过程中的生长和产量，如细菌挤奶。本研究的目的是调查在四氢嘧啶分泌生物体的膜流动性和完整性的变化引起的环境压力。对嗜盐四氢嘧啶产生菌Alkalibacillushalalalkaliphilus和嗜盐嗜盐嗜铬盐菌Chromohalibacillussalexigens在不同温度下进行了低温和高温处理。荧光标记的细胞的稳态各向异性进行测量，并通过流式细胞术和四氢嘧啶分布评估膜的完整性。强渗透下行冲击轻微增加了细菌膜的流动性。随着温度的升高，增加的膜流动性鼓励更多的四氢嘧啶释放在相同的渗透压休克条件下。另一方面，联合休克处理增加了解体细胞的数量。从四氢嘧啶释放和膜完整性的测量耦合热和渗透压休克条件下，我们可以优化两种细菌的分泌条件。
In response to sudden decrease in osmotic pressure, halophilic microorganisms secrete their accumulated osmolytes. This specific stress response, combined with physiochemical responses to the altered environment, influence the membrane properties and integrity of cells, with consequent effects on growth and yields in bioprocesses, such as bacterial milking. The aim of this study was to investigate changes in membrane fluidity and integrity induced by environmental stress in ectoine-secreting organisms. The halophilic ectoine-producing strainsAlkalibacillus haloalkaliphilusandChromohalobacter salexigenswere treated hypo- and hyper-osmotically at several temperatures. The steady-state anisotropy of fluorescently labeled cells was measured, and membrane integrity assessed by flow cytometry and ectoine distribution. Strong osmotic downshocks slightly increased the fluidity of the bacterial membranes. As the temperature increased, the increasing membrane fluidity encouraged more ectoine release under the same osmotic shock conditions. On the other hand, combined shock treatments increased the number of disintegrated cells. From the ectoine release and membrane integrity measurements under coupled thermal and osmotic shock conditions, we could optimize the secretion conditions for both bacteria.
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