Peritoneal Fluid and Tracer Albumin Kinetics in the Rat. Effects of Increases in Intraperitoneal Hydrostatic Pressure
Peritoneal Fluid and Tracer Albumin Kinetics in the Rat. Effects of Increases in Intraperitoneal Hydrostatic Pressure
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大鼠腹膜液和示踪白蛋白动力学。
DOI：
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发表时间：
1995
影响因子：
2.8
通讯作者：
B. Rippe
中科院分区：
文献类型：
--
作者：
E. Zakaria;B. Rippe
目的研究急性腹膜透析（PD）时腹腔液体丢失率、放射性示踪白蛋白（RISA）清除率（CI）及RISA清除率（CI -+ P）的变化。实验研究在麻醉Wistar大鼠。方法采用RISA稀释法测定腹腔内容量（IPV），并对PC中的RISA CI进行校正。使用量筒和预先称重的纱布组织获得留置结束时的容量恢复。分别通过透析液和血浆的重复微量采样获得CI和CI -+ P的测量值。连续测量IPP，并且可以通过使用充气袖带的外部同心腹部按压来改变IPP。在实验结束时，分析来自PC内衬组织的样品的RISA和水肿的含量，后者由湿/干重量比评估。结果1.36% Dianeal在2 mmHg的IPP（对照）下，RISA CI为27.1：1：2.0（：1：SE）μL·min-1，而CI→ P仅为8.07：1：0.67 μL·min-1，总液体丢失率为10.1：1：5.4μL·min-1。当IPP为14 mm Hg时，CI增加至55.3±4.1 μL·min-1，腹腔液吸收率为34.4±5.6 μL·min -1，而CI -+ P仅中度增加（11.2：1：1.4 μL·min-1）。min -1）。此外，在IPP升高时可检测到1.16：1：0.08 mL的胸腔积液。在2 mm Hg的IPP下，前腹肌（AAM）和膈肌（DIA）的水肿形成程度在150 min内基本上不显著，但在14 mm Hg时显著增加，AAM和DIA的RISA摄取也是如此。CI和CI -+ P之间的差异主要是由于示踪剂进入腹膜腔内衬组织，主要是AAM。结论：在毛细血管Starling平衡几乎不变的情况下，当IPPs增加时，PC的液体和RISA损失显著增加。然而，在IPP升高时，血浆中的RISA清除率似乎仅发生中度变化，仅占PC中RISA清除率的一小部分。在腹膜透析（PD）期间，PC内衬组织显然充当腹膜内蛋白质和液体的可变“水槽”。
Objectives To study the peritoneal fluid loss rate, the clearance (CI) of radioactive tracer albumin (RISA) eliminated from the peritoneal cavity (PC), as well as the peritoneal-to-plasma RISA clearance (CI -+ P) during acute peritoneal dialysis (PD) at large elevations in intraperitoneal hydrostatic pressure (IPP). Design Experimental study in anesthetized Wistar rats. Methods The intraperitoneal volume (IPV) was assessed using RISA dilution, correcting for the RISA CI from the PC. Volume recovery at termination of the dwells was obtained using graduated cylinders and preweighed gauze tissues. Measurements of CI and CI -+ P were obtained by repeated micro-sampling of dialysate and plasma, respectively. The IPP was continuously measured, and could be varied by external concentric abdominal compression using an inflatable cuff. On termination of the experiments, samples from tissues lining the PC were analyzed with respect to their content of RISA and edema, the latter being assessed from wet/dry weight ratios. Results At 2 mm Hg of IPP (control) the RISA CI was 27.1:1:2.0(:1:SE)μL.min-l, whereas CI→ Pwasonly 8.07:1:0.67 μL.min-l, at a total fluid loss rate of 10.1:1:5.4μL.min-1 for 1.36% Dianeal. At an IPP of 14 mm Hg, the CI increased to 55.3±4.1 μL.min -1 and the peritoneal fluid absorption rate was 34.4±5.6 μL.min -l, whereas CI -+ P was just moderately increased as compared to control (11.2:1:1.4 μL. min -I). Furthermore, a pleural effusion of 1.16:1:0.08 mL was detectable at elevated IPPs. The degree of edema formation in the anterior abdominal muscles (AAM) and the diaphragm (DIA) was largely insignificant during 150 min at 2 mm Hg of IPP, but increased markedly at 14 mm Hg, as did the RISA uptake to the AAM and DIA. The discrepancy between CI and CI -+ P was largely accounted for by tracer entrance into tissues lining the peritoneal cavity, mainly the AAM. Conclusions At a nearly unchanging capillary Starling equilibrium, the losses of fluid and of RISA from the PC were markedly elevated at increased IPPs. However, the RISA clearance to the plasma appeared to be only moderately altered at elevated IPP and represented only a minor fraction of the RISA clearance out of the PC. Tissues lining the PC apparently act as a variable ‘sink’ for intraperitoneal proteins and fluid during peritoneal dialysis (PD).
影响因子：
3.1
作者：
Rippe,B;Perry,MA;Granger,DN
通讯作者：
Granger,DN
小鼠腹膜吸收的淋巴和非淋巴途径：生理学与病理学。
影响因子：
3
作者：
Nagy,JA
通讯作者：
Nagy,JA