Improvement in bioactive protein storage stability and colon-targeted release: a simple double-layer chitosan-based particle
Improvement in bioactive protein storage stability and colon-targeted release: a simple double-layer chitosan-based particle
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生物活性蛋白储存稳定性和结肠靶向释放的改善：简单的双层壳聚糖颗粒
DOI：
10.1080/02652048.2019.1646336
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发表时间：
2019-07
影响因子：
3.9
通讯作者：
Wenbei Situ
中科院分区：
文献类型：
--
作者：
Wenbei Situ
摘要目的：为了提高生物活性蛋白的长期储存稳定性和随后的结肠靶向释放，开发了双层壳聚糖（CS）基颗粒。研究方法：为了形成双层CS基颗粒，将第二层交联到单层牛血清白蛋白（BSA）负载的CS基颗粒上。进一步研究了粒子的结构和性能。结果如下：随着第二层，双层颗粒变得更加紧凑，这对于通过强静电相互作用和水凝胶的溶胀来抑制生物活性蛋白在储存期间的泄漏是重要的。储存30 d后，C15-TPP/C15-HMP双层微粒中BSA的渗漏率仅为43.74-49.32%。此外，储存后在随后的结肠靶向递送中BSA的释放为44.02- 48.59%。结论：通过双层屏蔽和更紧凑的布置，可以减少生物活性蛋白在长期储存中的泄漏，并实现随后的结肠靶向递送。
Abstract Aim: To improve the long-term storage stability of a bioactive protein and the subsequent colon-targeted release, a double-layer chitosan (CS)-based particle was developed. Methods: To form the double-layer CS-based particle, the second layer was crosslinked onto the single-layer bovine serum albumin (BSA)-loaded CS-based particle. The structure and properties of particles were further investigated. Results: With the second layer, the double-layer particles became more compact, which was important for the inhibition of bioactive protein leakage during storage through strong electrostatic interactions and swelling of the hydrogels. After 30 d of storage, there was only 43.74–49.32% BSA leakage from the C15-TPP/C15-HMP double-layer particles. Moreover, the BSA release in subsequent colon-targeted delivery after storage was 44.02–48.59%. Conclusions: With double-layer shielding and a more compact arrangement, it was possible to reduce bioactive protein leakage over long periods storage and achieve subsequent colon-targeted delivery.
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作者：
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DOI：
10.1016/j.ijbiomac.2018.05.208
发表时间：
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影响因子：
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作者：
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