Sequence motifs and prokaryotic expression of the reptilian paramyxovirus fusion protein.
Sequence motifs and prokaryotic expression of the reptilian paramyxovirus fusion protein.
复制标题
DOI：
10.1007/s00705-005-0663-1
复制
发表时间：
2006-03
影响因子：
2.7
通讯作者：
Winton JR
中科院分区：
文献类型：
--
作者：
Franke J;Batts WN;Ahne W;Kurath G;Winton JR
选择了14种爬行动物副粘病毒分离物来代表这一新型病毒群中已知的遗传多样性程度。对选定的融合(F)基因区域进行测序、分析和比较。所有分离株的F基因均含有与副粘病毒科其他成员同源的保守基序，包括信号肽、跨膜结构域、furin切割位点、融合肽、n -连接糖基化位点和两个七核苷酸重复序列，其中第二个七核苷酸重复序列（HRB-LZ）具有亮氨酸拉链的特征。将Gono-GER85分离物融合基因的选定区域插入原核表达系统中，生成三个不同大小的重组蛋白片段。最长的重组蛋白被furin切割成两个预测长度的片段。用病毒中和兔抗血清对该分离物进行Western blot分析表明，只有最长的构建体与抗血清发生反应。该结构的独特之处在于含有30个额外的c端氨基酸，其中包括大部分HRB-LZ。这些结果表明，爬行类副粘病毒的F基因含有与其他爬行类副粘病毒家族成员相同的高度保守的基序，并提示爬行类副粘病毒F蛋白的HRB-LZ结构域含有一个线性抗原表位。
Fourteen reptilian paramyxovirus isolates were chosen to represent the known extent of genetic diversity among this novel group of viruses. Selected regions of the fusion (F) gene were sequenced, analyzed and compared. The F gene of all isolates contained conserved motifs homologous to those described for other members of the family Paramyxoviridae including: signal peptide, transmembrane domain, furin cleavage site, fusion peptide, N-linked glycosylation sites, and two heptad repeats, the second of which (HRB-LZ) had the characteristics of a leucine zipper. Selected regions of the fusion gene of isolate Gono-GER85 were inserted into a prokaryotic expression system to generate three recombinant protein fragments of various sizes. The longest recombinant protein was cleaved by furin into two fragments of predicted length. Western blot analysis with virus-neutralizing rabbit-antiserum against this isolate demonstrated that only the longest construct reacted with the antiserum. This construct was unique in containing 30 additional C-terminal amino acids that included most of the HRB-LZ. These results indicate that the F genes of reptilian paramyxoviruses contain highly conserved motifs typical of other members of the family and suggest that the HRB-LZ domain of the reptilian paramyxovirus F protein contains a linear antigenic epitope.
登录
查看更多内容
DOI：
10.1016/s0147-9571(99)00018-1
发表时间：
2000-01-01
影响因子：
2
作者：
Ahne, W;Mayr, A
通讯作者：
Mayr, A
影响因子：
3.8
作者：
BUCKLAND, R;MALVOISIN, E;WILD, F
通讯作者：
WILD, F
影响因子：
5
作者：
Franke, J;Essbauer, S;Blahak, S
通讯作者：
Blahak, S
影响因子：
3.8
作者：
CHAMBERS, P;PRINGLE, CR;EASTON, AJ
通讯作者：
EASTON, AJ
影响因子：
56.9
作者：
COHEN, C;PARRY, DAD
通讯作者：
PARRY, DAD