Al-induced secretion of organic acid, gene expression and root elongation in soybean roots
Al-induced secretion of organic acid, gene expression and root elongation in soybean roots
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铝诱导大豆根系有机酸分泌、基因表达和根伸长
DOI：
10.1007/s11738-012-1067-y
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发表时间：
2012
影响因子：
2.6
通讯作者：
Shen Hong
中科院分区：
文献类型：
--
作者：
Sasaki Takayuki;Yamamoto Yoko;Wei Wenhui;Shen Hong
铝激活的有机酸转运蛋白基因（ALMT和MATE）以及质膜H⁺ - ATP酶基因（PHA）已知在几种作物中对有机酸分泌的调节有作用。然而，这些基因如何相互作用以调节（此处“org”可能是“organic acid secretion”即有机酸分泌的缩写，但原句不完整）仍不清楚。
Al-activated organic acid transporter genes (ALMTandMATE) and plasma membrane H+-ATPase gene (PHA) are known to contribute to the regulation of organic acid secretion in several crops. However, it remains unclear how these genes interact to modulate organic acid exudation in the same plant species. In this study, Al-induced expression of genes (GmALMT1,GmMATE1andGmPHA1), secretion of organic acid and root elongation were characterized in soybean roots. Results indicated that treatment with 50 μM Al activated the expression ofGmALMT1,GmMATE1andGmPHA1, and the exudation of citrate and malate significantly in apical 5 mm region of soybean seedlings, but inhibited root elongation by 57.8 %. The highest malate exudation rate and the maximal expression ofGmALMT1andGmPHA1were observed after 2 h of 50 μM Al treatment, while the corresponding values for citrate exudation rate andGmMATE1expression occurred at 8 h. The exudation of malate and citrate contributed to but could not recover Al-triggered root elongation. A root-split experiment indicated that Al-activated gene expression, organic acid secretion and root growth inhibition required the direct contact of Al3+. The removal of shoots in soybean seedlings decreased Al-activated gene expression by 26.1–40.5 %, and secretion of organic acid by 14.7–40.2 %. Furthermore, shoot excision aggravated Al-inhibited root elongation, indicating the existence of other Al tolerance mechanism except the exudation of organic acid. These results suggested that Al-activated expression ofGmPHA1-,GmMATE1-andGmALMT1-mediated exudation of malate and citrate, and shoots played an important role in Al toxicity resistance in soybean roots.
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DOI：
10.1111/j.1469-8137.2004.01261.x
发表时间：
2004-12
期刊：
The New phytologist
影响因子：
--
作者：
Toshihiro Watanabe;S. Jansen;M. Osaki
通讯作者：
Toshihiro Watanabe;S. Jansen;M. Osaki
影响因子：
4.3
作者：
Zhao, ZQ;Ma, JF;Takeda, K
通讯作者：
Takeda, K
影响因子：
3
作者：
Yokosho, Kengo;Yamaji, Naoki;Ma, Jian Feng
通讯作者：
Ma, Jian Feng
影响因子：
7.4
作者：
Shen, H;He, LF;Matsumoto, H
通讯作者：
Matsumoto, H
影响因子：
7.4
作者：
Ryan, Peter R.;Raman, Harsh;Delhaize, Emmanuel
通讯作者：
Delhaize, Emmanuel