Clock Gene Daily Profiles and Their Phase Relationship in the Rat Suprachiasmatic Nucleus Are Affected by Photoperiod
Clock Gene Daily Profiles and Their Phase Relationship in the Rat Suprachiasmatic Nucleus Are Affected by Photoperiod
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光周期对大鼠视交叉上核时钟基因每日分布及其相位关系的影响
DOI：
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发表时间：
2003
影响因子：
3.5
通讯作者：
H. Illnerová
中科院分区：
文献类型：
--
作者：
A. Sumová;M. Jáč;M. Sládek;I. Sauman;H. Illnerová
大鼠视交叉上核（SCN）是昼夜节律起搏器的一个部位，其节律性受日照长度的影响，即受光周期的影响。尽管已经遵循了各种节律性标记，但到目前为止，还没有研究光周期对大鼠SCN中时钟基因表达的影响。为了填补差距，并更好地了解SCN状态的光周期调制，大鼠保持在一个长的光周期下，每天16小时的光和8小时的黑暗（LD 16：8）或在一个短的LD 8：16的光周期下，和Per1，Cry1，Bmal1和时钟mRNA在黑暗中的每日配置文件进行了评估，通过原位杂交方法。光周期影响节律基因表达的相位、波形和幅度以及它们之间的相位关系。长光周期条件下Per1 mRNA的持续时间比短光周期条件下长，而Bmal1 mRNA的持续时间比短光周期条件下短。在两种光周期下，早晨和白天Per1和Cry1 mRNA的上升以及早晨Bmal1 mRNA的下降与早晨的光照起始密切相关。Per1、Cry1和Bmal1 mRNA的节律幅度在短光周期下大于长光周期下。此外，在短光周期下，Clock mRNA的日表达具有明显的节律性。总之，这些数据表明，整个复杂的分子时钟在大鼠SCN是光周期依赖性的，因此可能会根据不同的季节。
Rhythmicity of the rat suprachiasmatic nucleus (SCN), a site of the circadian pacemaker, is affected by daylength; that is, by the photoperiod. Whereas various markers of rhythmicity have been followed, so far there have been no studies on the effect of the photoperiod on the expression of the clock genes in the rat SCN. To fill the gap and to better understand the photoperiodic modulation of the SCN state, rats were maintained either under a long photoperiod with 16 h of light and 8 h of darkness per day (LD16:8) or under a short LD8:16 photoperiod, and daily profiles of Per1, Cry1, Bmal1 and Clock mRNA in darkness were assessed by in situ hybridization method. The photoperiod affected phase, waveform, and amplitude of the rhythmic gene expression as well as phase relationship between their profiles. Under the long period, the interval of elevated Per1 mRNA lasted for a longer and that of elevated Bmal1 mRNA for a shorter time than under the short photoperiod. Under both photoperiods, the morning and the daytime Per1 and Cry1 mRNA rise as well as the morning Bmal1 mRNA decline were closely linked to the morning light onset. Amplitude of Per1, Cry1, and Bmal1 mRNA rhythms was larger under the short than under the long photoperiod. Also, under the short photoperiod, the daily Clock mRNA profile exhibited a significant rhythm. Altogether, the data indicate that the whole complex molecular clockwork in the rat SCN is photoperiod dependent and hence may differ according to the season of the year.
大鼠视交叉上核是四季的时钟。
DOI：
10.1073/pnas.92.17.7754
发表时间：
1995
影响因子：
11.1
作者：
Sumová,A;Trávnícková,Z;Peters,R;Schwartz,WJ;Illnerová,H
通讯作者：
Illnerová,H