Enhanced Performance of Magnetic Graphene Oxide-Immobilized Laccase and Its Application for the Decolorization of Dyes.
Enhanced Performance of Magnetic Graphene Oxide-Immobilized Laccase and Its Application for the Decolorization of Dyes.
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DOI：
10.3390/molecules22020221
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发表时间：
2017-02-01
期刊：
影响因子：
--
通讯作者：
Xiao A
中科院分区：
文献类型：
--
作者：
Chen J;Leng J;Yang X;Liao L;Liu L;Xiao A
通过将氨基Fe 3 O 4纳米粒子共价键合到氧化石墨烯（GO）上，制备了磁性氧化石墨烯（MGO）纳米材料，并将其作为载体用于漆酶的固定化。通过透射电子显微镜（TEM）和振动样品磁强计（VSM）的MGO-漆酶进行了表征。与游离漆酶相比，MGO-漆酶具有更好的pH稳定性和热稳定性。最适pH和温度确定为pH 3.0和35 °C。磁分离后的漆酶具有良好的磁响应性和重复使用性。该漆酶经10次使用后仍保持59.8%的酶活。最终将MGO-漆酶用于染料溶液的脱色，对结晶紫（CV）、孔雀石绿绿色（MG）和亮绿色（BG）的脱色率分别达到CV的94.7%，MG的95.6%，BG的91.4%。实验结果表明，MGO-漆酶纳米材料对水溶液中的染料具有良好的催化脱色能力。与游离酶相比，MGO作为酶固定化载体可有效提高漆酶的利用率，促进漆酶的应用。
In this study, magnetic graphene oxide (MGO) nanomaterials were synthesized based on covalent binding of amino Fe3O4 nanoparticles onto the graphene oxide (GO), and the prepared MGO was successfully applied as support for the immobilization of laccase. The MGO-laccase was characterized by transmission electron microscopy (TEM) and a vibrating sample magnetometer (VSM). Compared with free laccase, the MGO-laccase exhibited better pH and thermal stabilities. The optimum pH and temperature were confirmed as pH 3.0 and 35 °C. Moreover, the MGO-laccase exhibited sufficient magnetic response and satisfied reusability after being retained by magnetic separation. The MGO-laccase maintained 59.8% activity after ten uses. MGO-laccase were finally utilized in the decolorization of dye solutions and the decolorization rate of crystal violet (CV), malachite green (MG), and brilliant green (BG) reached 94.7% of CV, 95.6% of MG, and 91.4% of BG respectively. The experimental results indicated the MGO-laccase nanomaterials had a good catalysis ability to decolorize dyes in aqueous solution. Compared with the free enzyme, the employment of MGO as enzyme immobilization support could efficiently enhance the availability and facilitate the application of laccase.
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影响因子：
2.8
作者：
Bilal, Muhammad;Asgher, Muhammad;Zhang, Xuehong
通讯作者：
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影响因子：
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作者：
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DOI：
10.3390/molecules21081044
发表时间：
2016-08-10
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
4.6
作者：
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通讯作者：
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