Catalytic Oxidation of Phenol and 2,4-Dichlorophenol by Using Horseradish Peroxidase Immobilized on Graphene Oxide/Fe₃O₄.
Catalytic Oxidation of Phenol and 2,4-Dichlorophenol by Using Horseradish Peroxidase Immobilized on Graphene Oxide/Fe₃O₄.
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氧化石墨烯/Fe3O4 固定化辣根过氧化物酶催化氧化苯酚和 2,4-二氯苯酚
DOI：
10.3390/molecules21081044
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发表时间：
2016-08-10
期刊：
影响因子：
--
通讯作者：
Tang H
中科院分区：
文献类型：
--
作者：
Chang Q;Huang J;Ding Y;Tang H
采用超声波辅助反向共沉淀法制备氧化石墨烯/Fe 3 O 4（GO/Fe 3 O 4）纳米粒子，并以1-乙基-3-（3-二甲氨基丙基）碳二亚胺（EDC）为交联剂将辣根过氧化物酶（HRP）共价固定在GO/Fe 3 O 4上。为了提高苯酚的去除效率和防止酶的失活，在该反应中加入了具有高度亲水性的聚乙二醇，因为降解聚合物的吸附能力比HRP强。结果表明，固定化酶对苯酚的去除率可达95%以上。在pH值、PEG/苯酚质量比、固定化酶初始浓度和H2 O2初始浓度范围内对催化条件进行了优化。固定在GO/Fe 3 O 4复合材料上的HRP在磁场作用下可以很容易地从反应液中分离出来并重复使用。
Graphene oxide/Fe3O4 (GO/Fe3O4) nanoparticles were synthesized by an ultrasonic-assisted reverse co-precipitation method, and then horseradish peroxidase (HRP) was covalently immobilized onto GO/Fe3O4 with 1-ethyl-3-(3-dimethyaminopropyl)carbodiimide (EDC) as a cross-linking agent. In order to enhance the phenol removal efficiency and prevent the inactivation of the enzyme, the polyethylene glycol with highly hydrophilicity was added in this reaction, because the adsorption capacity for the polymer by degradation was stronger than the HRP. The results showed that the immobilized enzyme removed over 95% of phenol from aqueous solution. The catalytic condition was extensively optimized among the range of pH, mass ratio of PEG/phenol as well as initial concentration of immobilized enzyme and H2O2. The HRP immobilized on GO/Fe3O4 composite could be easily separated under a magnetic field from the reaction solution and reused.
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