Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters
Increased inflammation with crude E. coli LPS protects against acute leptospirosis in hamsters
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粗制大肠杆菌 LPS 增加炎症可预防仓鼠急性钩端螺旋体病
DOI：
10.1080/22221751.2019.1710435
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发表时间：
2020-01
影响因子：
13.2
通讯作者：
Yongguo Cao
中科院分区：
文献类型：
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作者：
Wenlong Zhang;Xufeng Xie;Jiaqi Wang;Ning Song;Tianbao Lv;Dianjun Wu;Naisheng Zhang;Yongguo Cao
摘要钩端螺旋体病是一种世界性的人畜共患病，可引起急性肾损伤、肝脏疾病、出血性疾病，甚至死亡。该病的治疗在很大程度上依赖于抗生素的使用，但最近对钩端螺旋体病发病机制的研究表明，免疫调节也可能是该病的有效治疗方法。由于炎症的延迟与钩端螺旋体的高致病性相关，我们研究了通过使用TLR4激活剂LPS诱导炎症对钩端螺旋体病的影响。与我们的假设一致，LPS治疗通过增强仓鼠的炎症反应来预防钩端螺旋体病。LPS治疗组在感染过程中TLR2、TLR4、NLRP 3和炎症因子基因表达水平均高于对照组。虽然LPS处理组的NO和iNOS水平高于钩端螺旋体感染组，但LPS诱导的保护作用不是iNOS依赖的。LPS处理诱导的抗钩端螺旋体IgG水平高于单独感染钩端螺旋体。然后分析共刺激分子和成熟标志物的表达。结果表明，LPS处理后，CD40、CD80和CD86的表达均增强。我们的结果表明，大肠杆菌（E. coli）产生的脂多糖（LPS）诱导的炎症增加。大肠杆菌）保护仓鼠免受钩端螺旋体病。
ABSTRACT Leptospirosis is a worldwide zoonotic disease that causes acute kidney injury, liver disease, bleeding disorders, and even death. Treatment of the disease is largely dependent on the use of antibiotics, but recent studies on pathogenesis of leptospirosis have shown that immunomodulation may also be an effective treatment for this disease. Since the delay in inflammation correlates with higher pathogenicity of leptospira, we studied the effect of inducing inflammation on leptospirosis by using TLR4 activator LPS. In accordance with our hypothesis, treatment with LPS protected against leptospirosis by enhancing the inflammatory response in hamsters. The gene expression levels of TLR2, TLR4, NLRP3 and inflammatory factors were higher in LPS-treated group during leptospira infection in hamsters. Although the levels of NO and iNOS were higher in LPS-treated group than in Leptospira-infected group, the protective effect induced by LPS is iNOS-independent. Treatment with LPS induced higher anti-leptospira IgG level than infection with leptospira alone. Then, expressions of costimulatory molecules and maturation markers were analysed. The data showed that treatment with LPS enhanced the expression of CD40, CD80 and CD86. Our results indicate that increased inflammation induced by LPS derived from Escherichia coli (E. coli) protects against leptospirosis in hamsters.
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影响因子：
3.4
作者：
V. Thongboonkerd;W. Chiangjong;Putita Saetun;S. Sinchaikul;Shui-Tein Chen;U. Kositanont
通讯作者：
V. Thongboonkerd;W. Chiangjong;Putita Saetun;S. Sinchaikul;Shui-Tein Chen;U. Kositanont
影响因子：
6.7
作者：
Zhang, X.;Song, Y.;Deng, X.
通讯作者：
Deng, X.
DOI：
10.14321/waterpathogens.26
发表时间：
2019
期刊：
Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Water Pathogen Project)
影响因子：
--
作者：
C. Goarant;G. Trueba;Emilie Bierque;Roman Thibeaux;Benjamin Davis;A. de la Peña-Moctezuma
通讯作者：
C. Goarant;G. Trueba;Emilie Bierque;Roman Thibeaux;Benjamin Davis;A. de la Peña-Moctezuma
影响因子：
5.5
作者：
Beutler, B;Hoebe, K;Ulevitch, RJ
通讯作者：
Ulevitch, RJ
DOI：
10.1093/trstmh/trt112
发表时间：
2014-02-01
影响因子：
2.2
作者：
Soares, Luciane Marieta;Macedo, Julio Oliveira;Athanazio, Daniel Abensur
通讯作者：
Athanazio, Daniel Abensur