Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9.
Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9.
复制标题
DOI：
10.1080/13880209.2021.1967409
复制
发表时间：
2021-12
影响因子：
3.8
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Zhang H;Dong W;Li S;Zhang Y;Lv Z;Yang L;Jiang L;Wu T;Wang Y
红景天苷是从红景天中提取的一种化合物。（景天科），具有许多有益的病理作用。探讨红景天苷对呼吸机所致肺内皮功能障碍的影响。在体内，雄性ICR小鼠分为假手术组、通气组、红景天苷组和通气+红景天苷组。小鼠通气4 h，通气前腹腔注射红景天苷（50 mg/kg），地塞米松（5 mg/kg）作为阳性对照。在体外，小鼠肺血管内皮细胞（MLVECs）用红景天苷、MMP-9 siRNA和BAY 11 -7082（10 μM）处理，然后暴露于周期性牵张4 h。然后收集肺组织和MLVEC进行进一步分析。红景天苷预处理显著逆转血管内皮钙粘蛋白的表达循环牵张处理的MLVEC中的VE-钙粘蛋白和闭合小带-1（ZO-1）蛋白（0.46 ± 0.09 vs. 0.80 ± 0.14，0.49 ± 0.05 vs. 0.88 ± 0.08）和通气肺组织（0.56 ± 0.06 vs. 0.83 ± 0.46，0.49 ± 0.08 vs. 0.80 ± 0.12）。结果进一步表明，红景天苷抑制牵张的MLVEC中基质金属蛋白酶-9（MMP-9）的表达，而其表达的敲低恢复了VE-钙粘蛋白（0.37 ± 0.08vs.0.85 ± 0.74）和ZO-1（0.48 ± 0.08vs.0.81 ± 0.11）的表达水平。同时，红景天苷抑制NF-κB信号通路，减轻肺损伤。红景天苷保护牵张诱导的内皮屏障功能，改善通气后的肺损伤。因此，红景天苷可能是一种有前途的治疗MV诱导的肺损伤患者的药物。
Salidroside, a compound extracted from Rhodiola rosea L. (Crassulaceae), possesses many beneficial pathological effects. To explore the effect of salidroside on ventilator-induced lung endothelial dysfunction in vivo and in vitro. In vivo, male ICR mice were divided into sham, ventilation, salidroside, and ventilation plus salidroside groups. The mice were ventilated for 4 h, salidroside (50 mg/kg) was administrated intraperitoneally before ventilation, dexamethasone (Dex) (5 mg/kg) was used as a positive control. In vitro, mouse lung vascular endothelial cells (MLVECs) were treated with salidroside, MMP-9 siRNA, and BAY11-7082 (10 μM), and then exposed to cyclic stretch for 4 h. Afterward, lung tissues and MLVECs were collected for further analysis. Salidroside pre-treatment significantly reversed the expression of vascular endothelial cadherin (VE-cadherin) and zonula occluden-1 (ZO-1) proteins in cyclic stretch-treated MLVECs (0.46 ± 0.09 vs. 0.80 ± 0.14, 0.49 ± 0.05 vs. 0.88 ± 0.08) and ventilated lung tissues (0.56 ± 0.06 vs. 0.83 ± 0.46, 0.49 ± 0.08 vs. 0.80 ± 0.12). The results further indicated that salidroside inhibited the expression of matrix metalloproteinase-9 (MMP-9), whereas knockdown of its expression restored the expression levels of VE-cadherin (0.37 ± 0.08 vs. 0.85 ± 0.74) and ZO-1 (0.48 ± 0.08 vs. 0.81 ± 0.11) in stretched MLVECs. Meanwhile, salidroside inhibited the NF-κB signalling pathway and alleviated lung injury. Salidroside protected against stretch-induced endothelial barrier function, improving lung injury after ventilation. Thus, salidroside may be a promising therapeutic agent for patients with MV-induced lung injury.
登录
查看更多内容
影响因子：
2.7
作者：
Liu MW;Su MX;Qin LF;Liu X;Tian ML;Zhang W;Wang YH
通讯作者：
Wang YH
DOI：
10.1165/ajrcmb.25.6.4558f
发表时间：
2001-12-01
影响因子：
6.4
作者：
Foda, HD;Rollo, EE;Zucker, S
通讯作者：
Zucker, S
DOI：
10.1165/rcmb.2008-0179oc
发表时间：
2009-07-01
影响因子：
6.4
作者：
Lukkarinen, Heikki;Hogmalm, Anna;Bry, Kristina
通讯作者：
Bry, Kristina
白藜芦醇对肺内皮屏障的保护涉及通过 Nrf2 依赖性机制抑制 HMGB1 释放和 HMGB1 诱导的线粒体氧化损伤
影响因子：
7.4
作者：
Dong, Wen-Wen;Liu, Yu-Jian;Jiang, Lai
通讯作者：
Jiang, Lai
影响因子：
37.8
作者：
Lindsey, ML;Escobar, P;Spinale, FG
通讯作者：
Spinale, FG