Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique.
Iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique.
复制标题
通过稳定同位素标记技术评估中国成年人铁的生理需求
DOI：
10.1186/s12986-018-0262-2
复制
发表时间：
2018
影响因子：
4.5
通讯作者：
Yang L
中科院分区：
文献类型：
--
作者：
Cai J;Ren T;Zhang Y;Wang Z;Gou L;Huang Z;Wang J;Piao J;Yang X;Yang L
背景铁是人体必需的微量元素，但目前对铁的生理需要量的研究还很有限，尤其是在我国。而且大多数研究都是用放射性同位素示踪技术进行的，这对健康是有害的。方法2010年1月至2011年3月，在河北省石家庄市白求恩军医大学随机抽取44名符合条件的中国青年健康成人，其中19名纳入最终数据分析。在适应饮食和观察后，受试者静脉注射58 Fe。在第0天采集基线静脉血样和一般信息。还分别在第14、30、60、100、120、150、240、330、425、515、605、767、1155天采集静脉血样。血样经微波消解系统酸消解后，用MC-ICP-MS和原子吸收光谱法分别测定铁同位素丰度和总铁浓度。循环率（血铁占全身铁的比例）可以通过摄入量、背景含量和峰值同位素含量来计算。结果58 Fe丰度在第14天达到峰值，从第425天开始趋于稳定。平均循环率为84%，男女之间无显著性差异。雌性大鼠平均铁需要量为1101.68 μg/d，经体重校正后的平均需要量为20.69 μg/kg. d。男性平均铁需要量为959.9 μg/d，经体重校正后为14.04 μg/kg·d。结论本研究利用稳定同位素标记技术获得了中国成年人铁生理需要量的数据，试验注册本试验已在中国临床试验注册中心注册（否，请执行以下操作： ChiCTR-TRC-09000581 ).
BackgroundIron is a kind of essential trace mineral in the human body, while the studies on its physiological requirement are very limited recently, especially in China. And most studies were performed with the radioisotope tracer technique, which was harmful to health. This study aimed to first get the value of iron physiological requirements in Chinese adults assessed by the stable isotope labeling technique.MethodsForty-four eligible young Chinese healthy adults were randomly recruited from the Bethune Military Medical College (Shijiazhuang, Hebei, China) between January 2010 and March 2011, and 19 subjects were included in the final data analysis. After adaptive diets and observation, subjects received58Fe intravenously. The baseline venous blood sample and general information were collected on day 0. Venous blood samples were also collected on day 14, 30, 60, 100, 120, 150, 240, 330, 425, 515, 605, 767, 1155, respectively. The blood samples were acid digested by a Microwave Digestion System and then analyzed by the MC-ICP-MS and Atomic Absorption Spectroscopy to get the abundance of Fe isotopes and the total iron concentration respectively. The circulation rate (the proportion of blood iron to whole body iron) could be calculated by the intake amount, background content and the peak isotope content. When the abundance changed stably, the iron physiological requirement could be calculated by the iron loss in a period of time.ResultsThe abundance of58Fe reached its peak on day 14, and changed stably from day 425. The average circulation rate was 84%, with no significance difference between the 2 genders. The mean iron requirement in females was 1101.68 μg/d, and the mean requirement adjusted by body weight was 20.69 μg/kg.d. For males, the mean iron requirement was 959.9 μg/d, and the requirement adjusted by body weight was 14.04 μg/kg.d.ConclusionOur study has obtained the data about the iron physiological requirements of Chinese adults using stable isotope labeling technique, which could provide the basis for adjusting iron DRIs of Chinese people in the future.Trial registrationThe trial was registered at the Chinese Clinical Trial Registry (No: ChiCTR-TRC-09000581 ).
登录
查看更多内容
影响因子：
2.6
作者：
Kiudeliene, Rosita;Griniute, Rasa;Labanauskas, Liutauras
通讯作者：
Labanauskas, Liutauras
放射性铁及其在贫血中的代谢：其吸收，运输和利用。
DOI：
10.1084/jem.69.5.739
发表时间：
1939-04-30
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Hahn PF;Bale WF;Lawrence EO;Whipple GH
通讯作者：
Whipple GH
影响因子：
5.9
作者：
Lim KH;Riddell LJ;Nowson CA;Booth AO;Szymlek-Gay EA
通讯作者：
Szymlek-Gay EA
影响因子：
3.6
作者：
Szymlek-Gay, Ewa A.;Domellof, Magnus;Egli, Ines M.
通讯作者：
Egli, Ines M.
影响因子：
7.1
作者：
Hurrell, Richard;Egli, Ines
通讯作者：
Egli, Ines