A single-cell lung atlas of complement genes identifies the mesothelium and epithelium as prominent sources of extrahepatic complement proteins.
A single-cell lung atlas of complement genes identifies the mesothelium and epithelium as prominent sources of extrahepatic complement proteins.
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DOI：
10.1038/s41385-022-00534-7
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发表时间：
2022-05
影响因子：
8
通讯作者：
Bosmann, Markus
中科院分区：
文献类型：
--
作者：
Chaudhary, Neha;Jayaraman, Archana;Reinhardt, Christoph;Campbell, Joshua D.;Bosmann, Markus
为了了解补体系统在宿主防御和肺损伤中的功能二重性，更全面地了解其在肺部的局部产生以及年龄对补体产生的影响至关重要。在这里，我们通过计算分析来自健康年轻（3 个月）和老年 C57BL/6 小鼠（24 个月）和人类肺转录组的现有单细胞 RNA 测序数据，探索了补体基因的表达。我们表征了 48 个补体基因的分布。在小鼠 28 种不同的免疫和非免疫细胞类型中，间皮细胞表达最多数量的补体基因（例如 C1ra、C2、C3）和调节因子（例如 Serping1、Cfh）。 C5 在 II 型肺泡上皮细胞中丰富，C1q 在间质性肺巨噬细胞中丰富。年轻和年老小鼠之间的基因表达仅存在中等差异。在 57 种人类肺细胞类型中，间皮细胞表现出丰富的补体表达。小鼠和人类肺细胞之间的基因表达（例如 FCN1、CFI、C6、C7）也存在一些明显差异。我们的研究结果为正常肺部补体基因的表达模式提供了新的视角。这些发现强调了补体在组织特异性稳态和免疫中的潜在功能，并可能促进对其在肺部健康和疾病中的作用的机制理解。
To understand functional duality of the complement system in host defense and lung injury, a more comprehensive view of its localized production in the lung, and the impact of age on complement production are essential. Here, we explored the expression of complement genes through computational analysis of preexisting single cell RNA sequencing data from lung transcriptomes of healthy young (3 months) and old C57BL/6 mice (24 months), and humans. We characterized the distribution of 48 complement genes. Across 28 distinct immune and non-immune cell types in mice, mesothelial cells expressed the greatest number of complement genes (e.g., C1ra, C2, C3), and regulators (e.g., Serping1, Cfh). C5 was abundant in type II alveolar epithelial cells and C1q in interstitial lung macrophages. There were only moderate differences in gene expression between young and old mice. Among 57 human lung cell types, mesothelial cells showed abundant complement expression. A few differences in gene expression (e.g., FCN1, CFI, C6, C7) were also evident between mice and human lung cells. Our findings present a novel perspective on the expression patterns of complement genes in normal lungs. These findings highlight the potential functions of complement in tissue-specific homeostasis and immunity and may foster a mechanistic understanding of its role in lung health and disease.
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影响因子：
--
作者：
Bosmann, Markus;Ward, Peter A.
通讯作者：
Ward, Peter A.
影响因子：
64.8
作者：
Gillich A;Zhang F;Farmer CG;Travaglini KJ;Tan SY;Gu M;Zhou B;Feinstein JA;Krasnow MA;Metzger RJ
通讯作者：
Metzger RJ
影响因子：
16.6
作者：
Angelidis, Ilias;Simon, Lukas M.;Schiller, Herbert B.
通讯作者：
Schiller, Herbert B.
DOI：
10.1513/pats.200803-025hr
发表时间：
2008-09-15
期刊：
Proceedings of the American Thoracic Society
影响因子：
--
作者：
Franks, Teri J;Colby, Thomas V;Williams, Mary C
通讯作者：
Williams, Mary C
影响因子：
15.9
作者：
Barker, Kimberly A.;Etesami, Neelou S.;Mizgerd, Joseph P.
通讯作者：
Mizgerd, Joseph P.