Giant lungfish genome elucidates the conquest of land by vertebrates.
Giant lungfish genome elucidates the conquest of land by vertebrates.
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巨大的肺鱼基因组阐明了脊椎动物对陆地的征服。
DOI：
10.1038/s41586-021-03198-8
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发表时间：
2021-03
期刊：
影响因子：
64.8
通讯作者：
Schartl M
中科院分区：
文献类型：
--
作者：
Meyer A;Schloissnig S;Franchini P;Du K;Woltering JM;Irisarri I;Wong WY;Nowoshilow S;Kneitz S;Kawaguchi A;Fabrizius A;Xiong P;Dechaud C;Spaink HP;Volff JN;Simakov O;Burmester T;Tanaka EM;Schartl M
肺鱼属于叶鳍鱼（Sarcopelii），在泥盆纪时期，“征服”了陆地，并最终产生了包括人类在内的所有陆地脊椎动物。在这里，我们确定的染色体质量基因组的澳大利亚肺鱼（新角鲨forsteri），这是已知的任何动物的最大的基因组。这种基因组的巨大尺寸，比人类大14倍，主要归因于巨大的基因间区域和具有高重复含量（约90%）的内含子，其组成部分更像四足动物（主要包括长散布的核元件）而不是鳍鱼。肺鱼的基因组继续独立扩展（其转座因子仍然活跃），通过与蝾螈巨大基因组不同的机制。17条完全组装的肺鱼宏染色体与其他脊椎动物染色体保持同线性，所有微染色体与祖先脊椎动物核型保持保守的古代同源性。我们的基因组分析证实了以前的报告，肺鱼占据了关键的进化地位，作为最接近的生活亲属四足动物，强调了肺鱼的重要性，了解与terrestrialization相关的创新。肺鱼对陆地生活的预适应包括在它们的叶状鳍中获得类似肢体的发育基因表达，如hoxc13和sall1。与专性呼吸空气相关的基因（如肺表面活性剂和气味受体基因家族（编码参与检测空气中气味的蛋白质）的扩展）的进化和复制速率增加，有助于肺鱼的四足动物生物学。这些发现推进了我们对脊椎动物进化过程中这一重大转变的理解。肺鱼Neoceratodus fosteri的染色体质量基因组揭示了专性空气呼吸的发展和在叶状鳍中获得肢状基因表达，为脊椎动物进化中的水到陆地过渡提供了见解。
Lungfishes belong to lobe-fined fish (Sarcopterygii) that, in the Devonian period, ‘conquered’ the land and ultimately gave rise to all land vertebrates, including humans. Here we determine the chromosome-quality genome of the Australian lungfish (Neoceratodus forsteri), which is known to have the largest genome of any animal. The vast size of this genome, which is about 14× larger than that of humans, is attributable mostly to huge intergenic regions and introns with high repeat content (around 90%), the components of which resemble those of tetrapods (comprising mainly long interspersed nuclear elements) more than they do those of ray-finned fish. The lungfish genome continues to expand independently (its transposable elements are still active), through mechanisms different to those of the enormous genomes of salamanders. The 17 fully assembled lungfish macrochromosomes maintain synteny to other vertebrate chromosomes, and all microchromosomes maintain conserved ancient homology with the ancestral vertebrate karyotype. Our phylogenomic analyses confirm previous reports that lungfish occupy a key evolutionary position as the closest living relatives to tetrapods, underscoring the importance of lungfish for understanding innovations associated with terrestrialization. Lungfish preadaptations to living on land include the gain of limb-like expression in developmental genes such as hoxc13 and sall1 in their lobed fins. Increased rates of evolution and the duplication of genes associated with obligate air-breathing, such as lung surfactants and the expansion of odorant receptor gene families (which encode proteins involved in detecting airborne odours), contribute to the tetrapod-like biology of lungfishes. These findings advance our understanding of this major transition during vertebrate evolution. A chromosome-quality genome of the lungfish Neoceratodus fosteri sheds light on the development of obligate air-breathing and the gain of limb-like gene expression in lobed fins, providing insights into the water-to-land transition in vertebrate evolution.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
2.4
作者：
Camp, E;Hope, R;Lardelli, M
通讯作者：
Lardelli, M
DOI：
10.1073/pnas.2011078117
发表时间：
2020-12-01
影响因子：
11.1
作者：
Fernandez-Guerrero M;Yakushiji-Kaminatsui N;Lopez-Delisle L;Zdral S;Darbellay F;Perez-Gomez R;Bolt CC;Sanchez-Martin MA;Duboule D;Ros MA
通讯作者：
Ros MA
影响因子：
30.8
作者：
Braasch I;Gehrke AR;Smith JJ;Kawasaki K;Manousaki T;Pasquier J;Amores A;Desvignes T;Batzel P;Catchen J;Berlin AM;Campbell MS;Barrell D;Martin KJ;Mulley JF;Ravi V;Lee AP;Nakamura T;Chalopin D;Fan S;Wcisel D;Cañestro C;Sydes J;Beaudry FE;Sun Y;Hertel J;Beam MJ;Fasold M;Ishiyama M;Johnson J;Kehr S;Lara M;Letaw JH;Litman GW;Litman RT;Mikami M;Ota T;Saha NR;Williams L;Stadler PF;Wang H;Taylor JS;Fontenot Q;Ferrara A;Searle SM;Aken B;Yandell M;Schneider I;Yoder JA;Volff JN;Meyer A;Amemiya CT;Venkatesh B;Holland PW;Guiguen Y;Bobe J;Shubin NH;Di Palma F;Alföldi J;Lindblad-Toh K;Postlethwait JH
通讯作者：
Postlethwait JH