Radioactive 198Au-doped nanostructures with different shapes for in vivo analyses of their biodistribution, tumor uptake, and intratumoral distribution.
Radioactive 198Au-doped nanostructures with different shapes for in vivo analyses of their biodistribution, tumor uptake, and intratumoral distribution.
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DOI：
10.1021/nn406258m
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发表时间：
2014-05-27
期刊：
影响因子：
17.1
通讯作者：
Xia Y
中科院分区：
文献类型：
--
作者：
Black KC;Wang Y;Luehmann HP;Cai X;Xing W;Pang B;Zhao Y;Cutler CS;Wang LV;Liu Y;Xia Y
以Au纳米笼为例，我们最近证明了放射性~(198)Au可以被掺入到Au纳米结构的晶格中，通过测量~(198)Au衰变的γ辐射来简单而可靠地定量它们在体内的生物分布，并通过检测切伦科夫辐射来进行光学成像。在这里，我们扩展了这一策略的能力，合成了大小相似但形状不同的放射性198Au纳米结构，然后使用小鼠EMT6乳腺癌模型比较了它们的生物分布、肿瘤摄取和肿瘤内分布。具体地说，我们研究了金纳米球、纳米盘、纳米棒和立方体纳米笼。在聚乙二醇化后，放射性金纳米结构的水悬浮液被静脉注射到荷瘤小鼠体内，通过γ辐射测量它们的生物分布，同时使用切伦科夫辐射直接成像它们的肿瘤摄取。注射后24小时，金纳米球和纳米盘的肿瘤摄取率明显高于金纳米棒和纳米笼。此外，在切除后的肿瘤薄片上进行放射自显影成像，以确定纳米结构在肿瘤内的分布。虽然金纳米球和纳米盘都只在肿瘤表面观察到，但金纳米棒和纳米笼分布在整个肿瘤中。
With Au nanocages as an example, we recently demonstrated that radioactive 198Au could be incorporated into the crystal lattice of Au nanostructures for simple and reliable quantification of their in vivo biodistribution by measuring the γ radiation from 198Au decay and for optical imaging by detecting the Cerenkov radiation. Here we extend the capability of this strategy to synthesize radioactive 198Au nanostructures with a similar size but different shapes and then compare their biodistribution, tumor uptake, and intratumoral distribution using a murine EMT6 breast cancer model. Specifically, we investigated Au nanospheres, nanodisks, nanorods, and cubic nanocages. After PEGylation, an aqueous suspension of the radioactive Au nanostructures was injected into a tumor-bearing mouse intravenously, and their biodistribution was measured from the γ radiation while their tumor uptake was directly imaged using the Cerenkov radiation. Significantly higher tumor uptake was observed for the Au nanospheres and nanodisks relative to the Au nanorods and nanocages at 24 h postinjection. Furthermore, autoradiographic imaging was performed on thin slices of the tumor after excision to resolve the intratumoral distributions of the nanostructures. While both the Au nanospheres and nanodisks were only observed on the surfaces of the tumors, the Au nanorods and nanocages were distributed throughout the tumors.
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DOI：
10.1007/s00259-013-2405-z
发表时间：
2013-08-01
影响因子：
9.1
作者：
Champion, Laurence;Lerebours, Florence;Alberini, Jean-Louis
通讯作者：
Alberini, Jean-Louis
DOI：
10.1016/j.jconrel.2013.09.013
发表时间：
2013-12-28
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Ernsting MJ;Murakami M;Roy A;Li SD
通讯作者：
Li SD
影响因子：
3.7
作者：
Doshi N;Mitragotri S
通讯作者：
Mitragotri S
DOI：
10.1021/la301279v
发表时间：
2012-06-12
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Kersey FR;Merkel TJ;Perry JL;Napier ME;DeSimone JM
通讯作者：
DeSimone JM
影响因子：
78.8
作者：
Jain, Rakesh K.;Stylianopoulos, Triantafyllos
通讯作者：
Stylianopoulos, Triantafyllos