Single‐fluorophore imaging with an unmodified epifluorescence microscope and conventional video camera
Single‐fluorophore imaging with an unmodified epifluorescence microscope and conventional video camera
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使用未经修改的落射荧光显微镜和传统摄像机进行单荧光团成像
DOI：
10.1046/j.1365-2818.1999.00482.x
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发表时间：
1999
影响因子：
2
通讯作者：
Ando
中科院分区：
文献类型：
--
作者：
Adachi;Kinosita JR;Ando
使用连接到未经修改的商用显微镜（IX70，奥林巴斯）的传统硅增强目标摄像机对水溶液中的单个荧光团进行实时成像，并用高压汞灯进行落射荧光激发。既不需要强大的激光，也不需要极其灵敏的摄像机。使用三个实验系统定量地证明单个、移动或静止的 Cy3 荧光团可以用显微镜成像：Cy3-凝溶胶蛋白附着在在重meromyosin上滑动的肌动蛋白丝上，滑动肌动蛋白丝稀疏地用Cy3标记，以及用一个或两个Cy3荧光团标记的重meromyosin。研究结果应该鼓励许多实验室尝试“单分子生理学”，即在生物机器完全活跃的环境中，在单分子水平上研究分子机器的功能和机制。
Single fluorophores in aqueous solution were imaged in real time with a conventional silicon‐intensified target video camera connected to an unmodified commercial microscope (IX70, Olympus) with epifluorescence excitation with a high‐pressure mercury lamp. Neither a powerful laser nor an extremely sensitive video camera was required. Three experimental systems were used to demonstrate quantitatively that individual, moving or stationary Cy3 fluorophores could be imaged with the microscope: Cy3‐gelsolin attached to an actin filament sliding over heavy meromyosin, sliding actin filaments sparsely labelled with Cy3, and heavy meromyosin labelled with one or two Cy3 fluorophores. The results should encourage many laboratories to attempt ‘single‐molecule physiology’ in which the functions and mechanisms of molecular machines are studied at the single‐molecule level in an environment where the biological machines are fully active.
影响因子：
56.9
作者：
Perkins, TT;Smith, DE;Chu, S
通讯作者：
Chu, S
DOI：
10.1002/cyto.990100103
发表时间：
1989-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
ERNST, LA;GUPTA, RK;WAGGONER, AS
通讯作者：
WAGGONER, AS