Transcriptionally active chromatin is sensitive to Neurospora crassa and S1 nucleases.
Transcriptionally active chromatin is sensitive to Neurospora crassa and S1 nucleases.
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转录活性染色质对粗糙脉孢菌和 S1 核酸酶敏感。
DOI：
10.1016/0022-2836(84)90076-7
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发表时间：
1984
影响因子：
5.6
通讯作者：
P. Schedl
中科院分区：
文献类型：
--
作者：
S. Han;A. Udvardy;P. Schedl
本文研究了黑腹果蝇87A7热休克基因座染色质中粗面脉孢菌和S1核酸酶裂解产物的分布。这两种核酸酶都在87A7基因座的特定位置产生染色质的单链和双链断裂。在热诱导前，我们发现两个87A7 HSP70基因的5‘端含有N。Crosa和S1核酸酶敏感部位，而只有几个来自该位点其他地方的切割产物。带着……粗核酸酶，我们观察到一个主要的5‘片段，这是来自DNA片段的切割，从转录单位开始，大约有90到115个碱基对。用S1核酸酶，我们发现了两个5‘端裂解产物。第一个基因从基因的起始处绘制了大约120到130个碱基对。有趣的是，这个位点对超螺旋DNA中的S1核酸酶也很敏感，但对线形裸露DNA不敏感。另一个片段与转录起始点非常接近(~0到−15个碱基对)。热诱导后，87A7的染色质结构发生转变。首先，显著的5‘N.crsa和S1核酸酶片段的产量明显减少。其次，整个HSP70基因，以及基因3‘端下游的间隔区DNA，对这两种核酸酶都变得高度敏感。
We have examined the distribution ofNeurospora crassaand S1nuclease cleavage products in the chromatin of the 87A7 heat shock locus ofDrosophila melanogaster. Both of these nucleases generate single and double-strand breaks in chromatin at specific sites in the 87A7 locus. Before heat induction, we find that the 5′ ends of the two 87A7 hsp 70 genes containN. crassaand S1nuclease hypersensitive sites, while there are only a few cleavage products from elsewhere in the locus. WithN. crassanuclease, we observe one major 5′ fragment, and this is derived from cleavage in a DNA segment mapping about 90 to 115 base-pairs from the beginning of the transcription unit. With S1nuclease, we find two 5′ cleavage products. The first maps about 120 to 130 base-pairs from the beginning of the gene. Interestingly, this site is also sensitive to S1nuclease in supercoiled but not linear naked DNA. The other fragment maps very close to the transcription start site (~0 to −15 base-pairs). After heat induction, there is a transition in the chromatin architecture of 87A7. First, there is a marked reduction in the yield of the prominent 5′N. crassaand S1nuclease fragments. Second, the entire hsp 70 gene, as well as the spacer DNA just downstream from the 3′ end of the gene, becomes highly sensitive to both of these nucleases.
影响因子：
5.6
作者：
Segraves,WA;Louis,C;Tsubota,S;Schedl,P;Rawls,JM;Jarry,BP
通讯作者：
Jarry,BP