ZmNF-YB16 Overexpression Improves Drought Resistance and Yield by Enhancing Photosynthesis and the Antioxidant Capacity of Maize Plants.
ZmNF-YB16 Overexpression Improves Drought Resistance and Yield by Enhancing Photosynthesis and the Antioxidant Capacity of Maize Plants.
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ZmNF-YB16 过表达通过增强玉米植物的光合作用和抗氧化能力来提高抗旱性和产量
DOI：
10.3389/fpls.2018.00709
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Wang B;Li Z;Ran Q;Li P;Peng Z;Zhang J
ZmNF-YB16是玉米NF-YB超家族的基本成员，是由NF-YA、NF-YB和NF-YC组成的转录因子复合体的成员。将ZmNF-YB16转化到自交系B104中，获得纯合子过表达系。ZmNF-YB16过表达在正常和干旱胁迫条件下，通过维持较高的光合作用，提高玉米植株在营养和生殖阶段的脱水和抗旱性，提高玉米产量。通过实时荧光定量PCR （qRT-PCR）检测野生型（WT）和转基因系之间的差异表达基因，发现ZmNF-YB16过表达增加了与内质网（ER）应激反应相关的抗氧化酶、抗氧化合成酶和分子伴侣基因的表达，改善了光合作用系统II的保护机制。过表达ZmNF-YB16的植株在对照和干旱胁迫条件下表现出更高的光合速率和抗氧化酶活性，更好的膜稳定性和更低的电解质泄漏。这些结果表明，ZmNF-YB16通过调控与光合作用、细胞抗氧化能力和内质网胁迫反应有关的多个基因的表达，在玉米抗旱性中发挥了重要作用。
ZmNF-YB16 is a basic NF-YB superfamily member and a member of a transcription factor complex composed of NF-YA, NF-YB, and NF-YC in maize. ZmNF-YB16 was transformed into the inbred maize line B104 to produce homozygous overexpression lines. ZmNF-YB16 overexpression improves dehydration and drought stress resistance in maize plants during vegetative and reproductive stages by maintaining higher photosynthesis and increases the maize grain yield under normal and drought stress conditions. Based on the examination of differentially expressed genes between the wild-type (WT) and transgenic lines by quantitative real time PCR (qRT-PCR), ZmNF-YB16 overexpression increased the expression of genes encoding antioxidant enzymes, the antioxidant synthase, and molecular chaperones associated with the endoplasmic reticulum (ER) stress response, and improved protection mechanism for photosynthesis system II. Plants that overexpression ZmNF-YB16 showed a higher rate of photosynthesis and antioxidant enzyme activity, better membrane stability and lower electrolyte leakage under control and drought stress conditions. These results suggested that ZmNF-YB16 played an important role in drought resistance in maize by regulating the expression of a number of genes involved in photosynthesis, the cellular antioxidant capacity and the ER stress response.
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DOI：
10.1073/pnas.0437909100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Lee, HS;Fischer, RL;Harada, JJ
通讯作者：
Harada, JJ
类囊体抗坏血酸过氧化物酶的过度表达显示番茄对冷胁迫的抵抗力增强
影响因子：
4.3
作者：
Duan, Ming;Feng, Hai-Long;Meng, Qing-Wei
通讯作者：
Meng, Qing-Wei
影响因子：
7.4
作者：
Jimenez, A;Hernandez, JA;Sevilla, F
通讯作者：
Sevilla, F
DOI：
10.1073/pnas.191389398
发表时间：
2001-09-25
影响因子：
11.1
作者：
Gaxiola, RA;Li, JS;Fink, GR
通讯作者：
Fink, GR
影响因子：
4.1
作者：
Coustry, F;Sinha, S;de Crombrugghe, B
通讯作者：
de Crombrugghe, B