Using hippocampal microRNA expression differences between mouse inbred strains to characterise miRNA function.
Using hippocampal microRNA expression differences between mouse inbred strains to characterise miRNA function.
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DOI：
10.1007/s00335-008-9116-y
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发表时间：
2008-08
期刊：
影响因子：
2.5
通讯作者：
Schalkwyk, Leonard C.
中科院分区：
文献类型：
--
作者：
Parsons, Michael J.;Grimm, Christina H.;Paya-Cano, Jose L.;Sugden, Karen;Nietfeld, Wilfried;Lehrach, Hans;Schalkwyk, Leonard C.
微RNA（micro-RNAs，miRNAs）是一类短的单链非编码RNA，在信使RNA（messenger RNA，mRNA）水平参与蛋白质编码基因的调控。它们参与许多性状的调节，包括发育时间、细胞凋亡、免疫功能和神经元发育。为了更好地理解miRNA本身的表达是如何调节的，我们在一个组织海马中寻找了四种小鼠近交系A/J、BALB/cJ、C57 BL/6 J和DBA/2 J之间的miRNA表达差异。共使用166个miRNA RT-PCR测定来筛选每个菌株的RNA池。使用每个菌株8个单独的样品进一步研究了菌株之间显著不同的20种miRNA种类，并且11种miRNA在菌株之间显示出显著差异（p < 0.05）。这是首次观察到不同近交系小鼠的miRNA表达差异。我们对这些差异表达的miRNA的表达与表型数据和mRNA表达进行了计算机相关分析，以更好地阐明这些miRNA对表型和mRNA表达调控的影响。这种方法使我们能够提名在焦虑，探索，学习和记忆中具有潜在作用的miRNAs。本文的在线版本（doi：10.1007/s 00335 -008-9116-y）包含补充材料，可供授权用户使用。
Micro-RNAs (miRNAs) are short, single-stranded, noncoding RNAs that are involved in the regulation of protein-coding genes at the level of messenger RNA (mRNA). They are involved in the regulation of numerous traits, including developmental timing, apoptosis, immune function, and neuronal development. To better understand how the expression of the miRNAs themselves is regulated, we looked for miRNA expression differences among four mouse inbred strains, A/J, BALB/cJ, C57BL/6J, and DBA/2J, in one tissue, the hippocampus. A total of 166 miRNA RT-PCR assays were used to screen RNA pools for each strain. Twenty miRNA species that were markedly different between strains were further investigated using eight individual samples per strain, and 11 miRNAs showed significant differences across strains (p < 0.05). This is the first observation of miRNA expression differences across inbred mice strains. We conducted an in silico correlation analysis of the expression of these differentially expressed miRNAs with phenotype data and mRNA expression to better characterise the effects of these miRNAs on both phenotype and the regulation of mRNA expression. This approach has allowed us to nominate miRNAs that have potential roles in anxiety, exploration, and learning and memory. The online version of this article (doi:10.1007/s00335-008-9116-y) contains supplementary material, which is available to authorized users.
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DOI：
10.1073/pnas.0700724104
发表时间：
2007-11-06
影响因子：
11.1
作者：
Guo, Yinglying;Lu, Peng;Peltz, Gary
通讯作者：
Peltz, Gary
影响因子：
64.8
作者：
Hovatta, I;Tennant, RS;Barlow, C
通讯作者：
Barlow, C
影响因子：
3.4
作者：
Fernandes, C;Paya-Cano, JL;Schalkwyk, LC
通讯作者：
Schalkwyk, LC
影响因子：
14.9
作者：
Betel D;Wilson M;Gabow A;Marks DS;Sander C
通讯作者：
Sander C
影响因子：
14.9
作者：
Griffiths-Jones S;Grocock RJ;van Dongen S;Bateman A;Enright AJ
通讯作者：
Enright AJ