Self-assembly of left- and right-handed molecular screws.
Self-assembly of left- and right-handed molecular screws.
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DOI：
10.1021/ja4106545
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发表时间：
2013-12-18
影响因子：
15
通讯作者：
Nanda V
中科院分区：
文献类型：
--
作者：
Xu F;Khan IJ;McGuinness K;Parmar AS;Silva T;Murthy NS;Nanda V
立体选择性是从催化到自组装的生物分子过程的标志，其主要发生在同手性物种之间。然而，已观察到合成多肽的同手性和异手性复合物，其中立体选择性取决于分子间相互作用的细节。这就提出了一个问题，即是否存在关于立体选择性的一般规则。相互作用螺旋的几何脊槽模型表明，在这类结构中，异手性缔合通常是有利的。我们使用简化的分子螺旋-由具有环状脂肪族侧链的L-或D-脯氨酸组成的胶原蛋白肽三螺旋来测试该原理。计算的稳定性一样，相反的手三螺旋配对表明异特异性协会的偏好。混合左手和右手螺旋大大降低了溶解度，导致微米级的片状组件，其特征在于与原子力显微镜的肽长度厚。X射线散射测量的螺旋间距在这些片支持一个紧密的脊槽包装的左，右手三重螺旋。
Stereoselectivity is a hallmark of biomolecular processes from catalysis to self-assembly, which predominantly occur between homochiral species. However, both homochiral and heterochiral complexes of synthetic polypeptides have been observed where stereoselectivity hinges on details of intermolecular interactions. This raises the question whether general rules governing stereoselectivity exist. A geometric ridges-in-grooves model of interacting helices indicates that heterochiral associations should generally be favored in this class of structures. We tested this principle using a simplified molecular screw – a collagen peptide triple- helix composed of either L- or D-proline with a cyclic aliphatic side chain. Calculated stabilities of like- and opposite- handed triple-helical pairings indicated a preference for heterospecific associations. Mixing left and right-handed helices drastically lowered solubility, resulting in micron-scale sheet-like assemblies that are one peptide-length thick as characterized with atomic force microscopy. X-ray scattering measurements of inter-helical spacing in these sheets support a tight ridges-in-grooves packing of left and right-handed triple helices.
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影响因子：
15
作者：
Pentelute BL;Gates ZP;Tereshko V;Dashnau JL;Vanderkooi JM;Kossiakoff AA;Kent SB
通讯作者：
Kent SB
影响因子：
8
作者：
Berisio, R;Vitagliano, L;Zagari, A
通讯作者：
Zagari, A
影响因子：
15
作者：
Nanda, V;Rosenblatt, MM;DeGrado, WF
通讯作者：
DeGrado, WF
DOI：
10.1073/pnas.0800291105
发表时间：
2008-06-24
影响因子：
11.1
作者：
Cejas, Mabel A.;Kinnney, William A.;Maryanoff, Bruce E.
通讯作者：
Maryanoff, Bruce E.
影响因子：
2.9
作者：
Kar, Karunakar;Ibrar, Sajjad;Nanda, Vikas;Getz, Todd M.;Kunapuli, Satya P.;Brodsky, Barbara
通讯作者：
Brodsky, Barbara