Cortical bone mechanical properties are altered in an animal model of progressive chronic kidney disease.
Cortical bone mechanical properties are altered in an animal model of progressive chronic kidney disease.
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DOI：
10.1371/journal.pone.0099262
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Allen MR
中科院分区：
文献类型：
--
作者：
Newman CL;Moe SM;Chen NX;Hammond MA;Wallace JM;Nyman JS;Allen MR
慢性肾脏病（CKD）可导致皮质骨丢失和孔隙率增加，增加骨折风险。动物模型已经证实，这些变化损害了整个骨的机械性能。全骨测试的估计表明，材料性能受到负面影响，尽管组织水平的评估尚未进行。因此，本研究的目的是使用慢性肾脏病进行性发展的大鼠模型来检查不同长度尺度的皮质骨的变化。在30周龄时（肾功能降低约75%），将生殖成熟的雄性Cy/+大鼠与其正常同窝仔进行比较。皮质骨材料的性能通过参考点压痕（RPI）、原子力显微镜（AFM）、拉曼光谱和高效液相色谱（HPLC）进行评估。RPI测量结果显示，CKD动物骨的压痕距离增加（+18%）和第一循环能量耗散（+8%）均高于正常对照组，AFM压痕显示CKD动物骨的弹性模量值分布更广，高模量值和低模量值的比例均高于正常对照组。然而，组织成分、胶原形态和胶原交联不能解释这些差异。尽管导致这些力学差异的特定骨骼组织改变尚不清楚，但这些结果表明，这些动物的皮质骨材料特性发生了改变，可能导致CKD相关骨折风险增加。
Chronic kidney disease (CKD), which leads tocortical bone loss and increasedporosity,increases therisk of fracture. Animal models have confirmed that these changes compromise whole bone mechanical properties. Estimates from whole bone testing suggest that material properties are negatively affected, though tissue-level assessmentshavenot been conducted. Therefore, the goal of the present study was to examine changes in cortical bone at different length scales using a rat model with theprogressive development of CKD. At 30 weeks of age (∼75% reduction in kidney function), skeletally mature male Cy/+ rats were compared to their normal littermates. Cortical bone material propertieswere assessed with reference point indentation (RPI), atomic force microscopy (AFM), Raman spectroscopy,and high performance liquid chromatography (HPLC). Bones from animals with CKD had higher (+18%) indentation distance increase and first cycle energy dissipation (+8%) as measured by RPI.AFM indentation revealed a broader distribution of elastic modulus values in CKD animals witha greater proportion of both higher and lower modulus values compared to normal controls. Yet, tissue composition, collagen morphology, and collagen cross-linking fail to account for these differences. Though the specific skeletal tissue alterations responsible for these mechanical differences remain unclear, these results indicate that cortical bone material properties are altered in these animals and may contribute to the increased fracture risk associated with CKD.
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影响因子：
4
作者：
Jamal, S. A.;Cheung, A. M.;Lok, C. E.
通讯作者：
Lok, C. E.
DOI：
10.1038/ki.2009.188
发表时间：
2009-08-01
期刊：
Kidney international. Supplement
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.1
作者：
Hammond, Max A.;Gallant, Maxime A.;Burr, David B.;Wallace, Joseph M.
通讯作者：
Wallace, Joseph M.
影响因子：
6.2
作者：
Gueerri-Fernandez, Roberto C.;Nogues, Xavier;Diez-Perez, Adolfo
通讯作者：
Diez-Perez, Adolfo
影响因子：
6.2
作者：
Diez-Perez, Adolfo;Guerri, Roberto;Nogues, Xavier;Caceres, Enric;Jesus Pena, Maria;Mellibovsky, Leonardo;Randall, Connor;Bridges, Daniel;Weaver, James C.;Proctor, Alexander;Brimer, Davis;Koester, Kurt J.;Ritchie, Robert O.;Hansma, Paul K.
通讯作者：
Hansma, Paul K.