Microindentation for in vivo measurement of bone tissue mechanical properties in humans.
Microindentation for in vivo measurement of bone tissue mechanical properties in humans.
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DOI：
10.1002/jbmr.73
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发表时间：
2010-08
影响因子：
6.2
通讯作者：
Hansma, Paul K.
中科院分区：
文献类型：
--
作者：
Diez-Perez, Adolfo;Guerri, Roberto;Nogues, Xavier;Caceres, Enric;Jesus Pena, Maria;Mellibovsky, Leonardo;Randall, Connor;Bridges, Daniel;Weaver, James C.;Proctor, Alexander;Brimer, Davis;Koester, Kurt J.;Ritchie, Robert O.;Hansma, Paul K.
骨组织力学性能被认为是骨强度的关键组成部分，但其评估需要侵入性程序。在这里，我们验证了一种新的仪器，参考点压痕（RPI）仪器，用于测量这些组织特性在体内。RPI仪器通过将探针组件插入覆盖胫骨的皮肤进行骨微压痕测试（BMT），并在置换骨膜后，以2 Hz的频率施加20个压痕循环，每个循环的最大力为11 N。我们评估了27名女性骨质疏松相关骨折和8名年龄相当的对照。骨折患者测量的总压痕距离（46.0 ± 14对31.7 ± 3.3 µm，p = 0.008）和压痕距离增加（18.1 ± 5.6对12.3 ± 2.9 µm，p = 0.008）显著大于对照组。两次测量的受试者工作特征（ROC）曲线下面积分别为93.1%（95%置信区间[CI] 83.1-100）和90.3%（95% CI 73.2-100）。观察者间变异系数范围为8.7%至15.5%，该程序耐受性良好。在尸体人骨样本（n = 5）的单独研究中，裂纹扩展韧性和压痕距离增加相关（r =-0.9036，p = 0.018），压痕和实验骨折诱导的裂纹的扫描电子显微镜图像相似。我们的结论是，BMT，通过诱导微观骨折，直接测量骨组织水平的力学性能。该方法重复性好，临床应用可行。它可以精确地区分有无脆性骨折的患者，并可为临床医生和研究人员提供直接的体内测量骨组织对骨折的抵抗力。© 2010美国骨与矿物质研究学会。
Bone tissue mechanical properties are deemed a key component of bone strength, but their assessment requires invasive procedures. Here we validate a new instrument, a reference point indentation (RPI) instrument, for measuring these tissue properties in vivo. The RPI instrument performs bone microindentation testing (BMT) by inserting a probe assembly through the skin covering the tibia and, after displacing periosteum, applying 20 indentation cycles at 2 Hz each with a maximum force of 11 N. We assessed 27 women with osteoporosis-related fractures and 8 controls of comparable ages. Measured total indentation distance (46.0 ± 14 versus 31.7 ± 3.3 µm, p = .008) and indentation distance increase (18.1 ± 5.6 versus 12.3 ± 2.9 µm, p = .008) were significantly greater in fracture patients than in controls. Areas under the receiver operating characteristic (ROC) curve for the two measurements were 93.1% (95% confidence interval [CI] 83.1–100) and 90.3% (95% CI 73.2–100), respectively. Interobserver coefficient of variation ranged from 8.7% to 15.5%, and the procedure was well tolerated. In a separate study of cadaveric human bone samples (n = 5), crack growth toughness and indentation distance increase correlated (r = –0.9036, p = .018), and scanning electron microscope images of cracks induced by indentation and by experimental fractures were similar. We conclude that BMT, by inducing microscopic fractures, directly measures bone mechanical properties at the tissue level. The technique is feasible for use in clinics with good reproducibility. It discriminates precisely between patients with and without fragility fracture and may provide clinicians and researchers with a direct in vivo measurement of bone tissue resistance to fracture. © 2010 American Society for Bone and Mineral Research.
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影响因子：
2.4
作者：
Ascenzi, Maria-Grazia;Gill, Jaya;Lomovtsev, Alexander
通讯作者：
Lomovtsev, Alexander
影响因子：
2.4
作者：
Habelitz, S;Marshall, GW;Marshall, SJ
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Marshall, SJ
影响因子：
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作者：
Gupta, Himadri S.;Fratzl, Peter;Kirchner, Helmut O. K.
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影响因子：
41.2
作者：
Koester, K. J.;Ager, J. W., III;Ritchie, R. O.
通讯作者：
Ritchie, R. O.
影响因子：
9.1
作者：
Fantner, Georg E.;Rabinovych, Olexandr;Hansma, Paul K.
通讯作者：
Hansma, Paul K.