Overexpression of ZmAFB2, the maize homologue of AFB2 gene, enhances salt tolerance in transgenic tobacco

Overexpression of ZmAFB2, the maize homologue of AFB2 gene, enhances salt tolerance in transgenic tobacco
复制标题

ZmAFB2(AFB2 基因的玉米同源物)的过表达可增强转基因烟草的耐盐性

DOI:
10.1007/s11240-012-0219-5
复制
发表时间:
2013-02
期刊:
Plant Cell, Tissue and Organ Culture
影响因子:
--
通讯作者:
Li, Zhengguo
Li, Zhengguo
中科院分区:
其他
文献类型:
--
作者:
Yang, Chunwen;Deng, Wei;Tang, Ning;Wang, Xiaomin;Yan, Fang;Lin, Dongbo;Li, Zhengguo

文献摘要

参考文献

被引文献

相似文献

TIR 1/AFB F-box蛋白作为生长素受体,在生长素介导的植物发育过程中发挥重要作用。本研究从玉米(Zea mays L.)中克隆了黄曲霉毒素B2(AFB 2)基因的同源基因ZmAFB 2。NaCl和水杨酸处理诱导ZmAFB 2的表达,吲哚乙酸(IAA)处理降低ZmAFB 2的表达。为了研究ZmAFB 2基因在逆境条件下的功能,构建了由CaMV 35 S启动子驱动的ZmAFB 2双元表达载体,并通过农杆菌介导法转化烟草(Nicotiana tabacum)。过量表达ZmAFB 2的转基因烟草表现出对盐处理的耐受性增加。此外,ZmAFB 2的过表达增强了胁迫响应基因抗坏血酸过氧化物酶和过氧化氢酶的转录水平。这些结果表明,ZmAFB 2参与了非生物胁迫反应,ZmAFB 2的过表达增强了转基因烟草的耐盐性。
The TIR1/AFB F-box proteins act as auxin receptors and play an important role in auxin mediated plant development. In the present study, a homologue of AFB2 gene, ZmAFB2, has been cloned from maize (Zea mays L.). Expression of ZmAFB2 is induced by NaCl and salicylic acid treatment and reduced by indole-3-acetic acid (IAA) treatment. To identify the function of the ZmAFB2 gene under stress conditions, a binary vector containing ZmAFB2 driven by CaMV 35S promoter was constructed and transformed into tobacco (Nicotiana tabacum) by Agrobacterium-mediated transformation. The transgenic tobacco lines overexpressing ZmAFB2 exhibit increased tolerance to salt treatment. Furthermore, overexpression of ZmAFB2 enhances the transcription levels of stress responsive genes Ascorbate peroxidase and Catalase. These results indicate that ZmAFB2 is involved in abiotic stress response and overexpression of ZmAFB2 enhances salt tolerance in transgenic tobacco.
DOI: 10.1134/s1021443711010213
发表时间: 2011-01
期刊: Russ J Plant Physiol
影响因子: --
作者:
杨辉;田云;李培旺;青先国;张操昊;卢向阳;方俊
通讯作者: 方俊
DOI: 10.1371/journal.ppat.0020123
发表时间: 2006-11
期刊: PLOS PATHOGENS
影响因子: 6.7
作者:
Wang, Dong;Amornsiripanitch, Nita;Dong, Xinnian
通讯作者: Dong, Xinnian
DOI: 10.1023/b:grow.0000007504.41476.c2
发表时间: 2003-11
影响因子: 4.2
作者:
Esen Taşğın;Ö. Atici;B. Nalbantoğlu
通讯作者: Esen Taşğın;Ö. Atici;B. Nalbantoğlu
DOI: 10.1007/s11240-011-0064-y
发表时间: 2012-02
期刊: Plant Cell, Tissue and Organ Culture (PCTOC)
影响因子: --
作者:
Wen-Lii Huang;Chia-Hui Lee;Yi-Rung Chen
通讯作者: Wen-Lii Huang;Chia-Hui Lee;Yi-Rung Chen
DOI: 10.1105/tpc.022574
发表时间: 2004-07-01
期刊: PLANT CELL
影响因子: 11.6
作者:
Goodman, CD;Casati, P;Walbot, V
通讯作者: Walbot, V