Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum

Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum
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烟草转运抑制剂反应 1 (TIR1) 的分子克隆和表征

DOI:
10.1134/s1021443711010213
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发表时间:
2011-01
期刊:
Russ J Plant Physiol
影响因子:
--
通讯作者:
方俊
方俊
中科院分区:
其他
文献类型:
--
作者:
杨辉;田云;李培旺;青先国;张操昊;卢向阳;方俊

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通过cDNA末端快速扩增(RACE)方法首次从烟草中分离出编码转运抑制反应1(TIR1)蛋白的全长cDNA,命名为NtTIR1。 NtTIR1 包含一个 1746 bp 的开放阅读框,编码 581 个氨基酸。推导的 NtTIR1 蛋白与其他双子叶植物的 TIR1 蛋白具有较高的同源性,计算分子量为 65.2 kD,理论等电点值为 6.02,预计具有 F-box 结构域。生物信息学分析表明,推导的NtTIR1包含三个跨膜结构域,预测的NtTIR1 3D模型具有拟南芥TIR1蛋白的典型空间结构。转录模式分析表明NtTIR1的转录是由IAA和ABA诱导的。
The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA.
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