Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum
Molecular cloning and characterization of a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) from Nicotiana tabacum
复制标题
烟草转运抑制剂反应 1 (TIR1) 的分子克隆和表征
DOI:
10.1134/s1021443711010213
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发表时间:
2011-01
期刊:
影响因子:
--
通讯作者:
方俊
中科院分区:
文献类型:
--
作者:
杨辉;田云;李培旺;青先国;张操昊;卢向阳;方俊
The full-length cDNA encoding a TRANSPORT INHIBITOR RESPONSE 1 (TIR1) protein, designated NtTIR1, was isolated for the first time from Nicotiana tabacum by the rapid amplification of cDNA ends (RACE) method. NtTIR1 contained a 1746-bp open reading frame encoding 581 amino acids. The deduced NtTIR1 protein, which showed high identity to TIR1 protein of other dicotyledonous plants, had a calculated mol wt of 65.2 kD and a theoretical pI value of 6.02 and was predicted to possess an F-box domain. Bioinformatic analyses revealed that the deduced NtTIR1 contained three transmembrane domains, and the predicted 3D model of NtTIR1 had a typical spatial structure of the TIR1 protein from Arabidopsis thaliana. Transcription pattern analysis revealed that the transcription of NtTIR1 was induced by IAA and ABA.
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DOI:
10.1111/j.1365-313x.2006.02934.x
发表时间:
2006-11
期刊:
The Plant journal : for cell and molecular biology
影响因子:
--
作者:
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影响因子:
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DOI:
10.1111/j.1365-3040.1994.tb00147.x
发表时间:
1994-05
期刊:
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影响因子:
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作者:
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通讯作者:
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影响因子:
5.8
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