Central Role of Core Binding Factor β2 in Mucosa-Associated Lymphoid Tissue Organogenesis in Mouse.
Central Role of Core Binding Factor β2 in Mucosa-Associated Lymphoid Tissue Organogenesis in Mouse.
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DOI：
10.1371/journal.pone.0127460
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Kiyono H
中科院分区：
文献类型：
--
作者：
Nagatake T;Fukuyama S;Sato S;Okura H;Tachibana M;Taniuchi I;Ito K;Shimojou M;Matsumoto N;Suzuki H;Kunisawa J;Kiyono H
粘膜相关淋巴组织（MALT）是一组发育在不同粘膜表面的继发性和有组织的淋巴组织。Peyer’s patches （PPs）、鼻咽相关淋巴组织（NALT）和泪管相关淋巴组织（TALT）分别是小肠、鼻腔和泪囊中具有代表性的MALT。最近的一项研究表明，核心结合因子(Cbf) β2和启动子1转录的runt相关转录因子1 （P1-Runx1）的转录调节因子是CD3 - CD4+CD45+淋巴组织诱导剂（LTi）细胞分化所必需的，这些细胞启动并触发了PPs的发育程序，但该途径在NALT和TALT发育中的作用仍有待阐明。在这里，我们报道了Cbfβ2通过调节LTi细胞向NALT和TALT的转运，在NALT和TALT的发展中发挥重要作用。Cbfβ2在三种类型MALT的LTi细胞中均有表达。事实上，与先前对PPs的发现相似，我们发现Cbfβ2−/−小鼠缺乏NALT和TALT淋巴样结构。然而，与PPs不同的是，在P1-Runx1或Runx2、Runx3等Runx家族成员缺失的情况下，NALT和TALT发育正常。在cbf - β2−/−小鼠中，用于NALT和TALT的LTi细胞正常分化，但在各自的淋巴组织色素中不积累。这些发现表明，Cbfβ2是MALT发育程序的中心调节因子，但Runx蛋白对淋巴组织发育的依赖性在PPs、NALT和TALT中有所不同。
Mucosa-associated lymphoid tissue (MALT) is a group of secondary and organized lymphoid tissue that develops at different mucosal surfaces. Peyer’s patches (PPs), nasopharynx-associated lymphoid tissue (NALT), and tear duct-associated lymphoid tissue (TALT) are representative MALT in the small intestine, nasal cavity, and lacrimal sac, respectively. A recent study has shown that transcriptional regulators of core binding factor (Cbf) β2 and promotor-1-transcribed Runt-related transcription factor 1 (P1-Runx1) are required for the differentiation of CD3−CD4+CD45+ lymphoid tissue inducer (LTi) cells, which initiate and trigger the developmental program of PPs, but the involvement of this pathway in NALT and TALT development remains to be elucidated. Here we report that Cbfβ2 plays an essential role in NALT and TALT development by regulating LTi cell trafficking to the NALT and TALT anlagens. Cbfβ2 was expressed in LTi cells in all three types of MALT examined. Indeed, similar to the previous finding for PPs, we found that Cbfβ2−/− mice lacked NALT and TALT lymphoid structures. However, in contrast to PPs, NALT and TALT developed normally in the absence of P1-Runx1 or other Runx family members such as Runx2 and Runx3. LTi cells for NALT and TALT differentiated normally but did not accumulate in the respective lymphoid tissue anlagens in Cbfβ2−/− mice. These findings demonstrate that Cbfβ2 is a central regulator of the MALT developmental program, but the dependency of Runx proteins on the lymphoid tissue development would differ among PPs, NALT, and TALT.
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影响因子：
3.7
作者：
Okura H;Sato S;Kishikawa S;Kaneto S;Nakashima T;Yoshida N;Takayanagi H;Kiyono H
通讯作者：
Kiyono H
影响因子：
4.4
作者：
Mebius, RE;Miyamoto, T;Akashi, K
通讯作者：
Akashi, K
影响因子：
11.4
作者：
Levanon, D;Bettoun, D;Groner, Y
通讯作者：
Groner, Y
影响因子：
20.3
作者：
Bee, Thomas;Swiers, Gemma;de Bruijn, Marella F. T. R.
通讯作者：
de Bruijn, Marella F. T. R.
影响因子：
64.5
作者：
Li, QL;Ito, K;Ito, Y
通讯作者：
Ito, Y