Role of peripheral myelin protein 22 in chronic exercise‐induced interactions of muscle and bone in mice
Role of peripheral myelin protein 22 in chronic exercise‐induced interactions of muscle and bone in mice
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外周髓磷脂蛋白 22 在小鼠慢性运动诱导的肌肉和骨骼相互作用中的作用
DOI：
10.1002/jcp.30706
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发表时间：
2022
影响因子：
5.6
通讯作者：
Kaji Hiroshi
中科院分区：
文献类型：
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作者：
Kawaguchi Miku;Kawao Naoyuki;Muratani Masafumi;Takafuji Yoshimasa;Ishida Masayoshi;Kinoshita Yuko;Takada Yuto;Mizukami Yuya;Ohira Takashi;Kaji Hiroshi
运动对预防和治疗骨质疏松症很重要。虽然骨骼肌和骨骼之间的相互作用最近已经有报道，但在运动过程中连接肌肉和骨骼的肌肉运动因子仍不清楚。我们之前发现，使用跑步机跑步的慢性运动可以钝化卵巢切除所致的小鼠骨量减少。在此，我们对有或没有慢性运动的雄性小鼠的腓肠肌和比目鱼肌进行了RNA序列分析，以确定导致慢性运动对肌肉/骨骼关系影响的肌肉因子。我们从RNA序列分析中提取了外周髓鞘蛋白22(PMP22)作为一种体液因子，该因子可能是慢性运动诱导的小鼠比目鱼肌和腓肠肌中的一种。慢性运动显著增强雌性小鼠腓肠肌和比目鱼肌中PMP22的表达。PMP22抑制巨噬细胞集落刺激因子和受体激活因子κB配体诱导的小鼠骨髓细胞破骨细胞相关基因表达和破骨细胞形成的增加。此外，PMP22显著抑制小鼠成骨细胞的分化、碱性磷酸酶活性和矿化；然而，PMP22的过表达并不影响小鼠肌肉C2C12细胞的肌肉表型。一项简单的回归分析显示，无论是否进行慢性运动，腓肠肌和比目鱼肌中PMP22的mRNA水平与股骨的皮质骨密度呈正相关。综上所述，我们确定PMP22是一种新的由慢性运动诱导的小鼠肌肉细胞因子。我们在体外首次发现PMP22抑制破骨细胞的形成和成骨细胞的表型。
Exercise is important for the prevention and treatment of sarcopenia and osteoporosis. Although the interactions between skeletal muscles and bone have recently been reported, the myokines linking muscle to bone during exercise remain unknown. We previously revealed that chronic exercise using treadmill running blunts ovariectomy‐induced osteopenia in mice. We herein performed an RNA sequence analysis of the gastrocnemius and soleus muscles of male mice with or without chronic exercise to identify the myokines responsible for the effects of chronic exercise on the muscle/bone relationship. We extracted peripheral myelin protein 22 (PMP22) as a humoral factor that was putatively induced by chronic exercise in the soleus and gastrocnemius muscles of mice from the RNA sequence analysis. Chronic exercise significantly enhanced the expression of PMP22 in the gastrocnemius and soleus muscles of female mice. PMP22 suppressed macrophage‐colony stimulating factor and receptor activator factor κB ligand‐induced increases in the expression of osteoclast‐related genes and osteoclast formation from mouse bone marrow cells. Moreover, PMP22 significantly inhibited osteoblast differentiation, alkaline phosphatase activity, and mineralization in mouse osteoblast cultures; however, the overexpression of PMP22 did not affect muscle phenotypes in mouse muscle C2C12 cells. A simple regression analysis revealed that PMP22 mRNA levels in the gastrocnemius and soleus muscles were positively related to cortical bone mineral density at the femurs of mice with or without chronic exercise. In conclusion, we identified PMP22 as a novel myokine induced by chronic exercise in mice. We first showed that PMP22 suppresses osteoclast formation and the osteoblast phenotype in vitro.
olfactomedin 1 在小鼠超重力诱导的肌肉和骨骼改变中的作用。
DOI：
--
发表时间：
2021
期刊：
影响因子：
--
作者：
Kawao N;Shimoide T;Morita H;Mizukami Y;Takafuji Y;Ishida M;Kaji H.
通讯作者：
Kaji H.
影响因子：
--
作者：
Ashki N;Gordon L;Wadehra M
通讯作者：
Wadehra M