Synergistic protection of astragalus polysaccharides and matrine against ulcerative colitis and associated lung injury in rats
Synergistic protection of astragalus polysaccharides and matrine against ulcerative colitis and associated lung injury in rats
复制标题
黄芪多糖和苦参碱对大鼠溃疡性结肠炎及相关肺损伤的协同保护作用
DOI：
10.3748/wjg.v26.i1.55
复制
发表时间：
2020-01
影响因子：
4.3
通讯作者：
Bai Guo-Min
中科院分区：
文献类型：
--
作者：
Yan Xin;Lu Qing-Ge;Zeng Li;Li Xiao-Hai;Liu Yu;Du Xue-Feng;Bai Guo-Min
背景溃疡性结肠炎（UC）是炎症性肠病的主要形式。由于UC病因复杂，复发率高，因此阐明其发病机制并寻找有效的治疗方法是非常必要的。目的观察黄芪多糖（APS）联合苦参碱对溃疡性结肠炎（UC）及相关肺损伤的影响。方法采用结肠黏膜组织致敏联合三硝基苯磺酸乙醇诱导大鼠UC模型。观察柳氮比林、黄芪多糖、苦参碱及黄芪多糖与苦参碱合用对肺、结肠组织病理学改变、疾病活动指数（DAI）、结肠黏膜损伤指数（CMDI）、血清内毒素（ET）水平、血清二胺氧化酶（DAO）活性、肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1β）含量、髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、检测UC大鼠肺组织丙二醛（MDA）含量及肺组织中闭锁小带（ZO）-1、闭锁蛋白（Occludin）和三叶因子3（TFF 3）的蛋白表达。结果柳氮比林、黄芪多糖、苦参碱及黄芪多糖与苦参碱联合治疗可降低UC大鼠DAI评分，改善结肠和肺组织病理学改变，降低CMDI评分、ET水平和DAO活性。柳氮比林、黄芪多糖、苦参碱及黄芪多糖与苦参碱联合应用均能减轻UC大鼠肺组织的炎症反应和氧化应激损伤。此外，ZO-1、Occludin和TFF 3在UC大鼠肺和结肠组织中的表达在不同治疗后增加。值得注意的是，APS联合苦参碱对UC和肺损伤的保护作用优于其他治疗。结论黄芪多糖联合苦参碱对UC及肺损伤具有协同保护作用，其机制可能与调节TFF 3表达有关。
BACKGROUND Ulcerative colitis (UC) is a main form of inflammatory bowel disease. Due to complicated etiology and a high rate of recurrence, it is quite essential to elucidate the underlying mechanism of and search for effective therapeutic methods for UC. AIM To investigate the effects of astragalus polysaccharides (APS) combined with matrine on UC and associated lung injury. METHODS UC was induced in rats by colon mucosal tissue sensitization combined with trinitro-benzene-sulfonic acid-ethanol. Then, the effects of the treatments of salazopyrine, APS, matrine, and APS combined with matrine on histopathological changes of lung and colon tissues, disease activity index (DAI), colon mucosal damage index (CMDI), serum endotoxin (ET) level, serum diamine oxidase (DAO) activity, the contents of tumor necrosis factor-α and interleukin-1β, and the activities of myeloperoxidase, superoxide dismutase, and malondialdehyde in lung tissues, as well as the protein expression of zonula occludens (ZO)-1, Occludin, and trefoil factor 3 (TFF3) were detected in UC rats. RESULTS The treatments of salazopyrine, APS, matrine, and APS combined with matrine reduced DAI scores and improved histopathological changes of colon and lung tissues, as well as decreased CMDI scores, ET levels, and DAO activities in UC rats. Moreover, in lung tissues, inflammatory response and oxidative stress injury were relieved after the treatments of salazopyrine, APS, matrine, and APS combined with matrine in UC rats. Furthermore, the expression of ZO-1, Occludin, and TFF3 in lung and colon tissues was increased after different treatments in UC rats. Notably, APS combined with matrine exerted a better protective effect against UC and lung injury compared with other treatments. CONCLUSION APS combined with matrine exert a synergistic protective effect against UC and lung injury, which might be associated with regulating TFF3 expression.
登录
查看更多内容
影响因子：
2.2
作者：
Laubach VE;Sharma AK
通讯作者：
Sharma AK
DOI：
10.1016/j.cgh.2017.06.016
发表时间：
2018-03
期刊：
Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association
影响因子：
--
作者：
Fumery M;Singh S;Dulai PS;Gower-Rousseau C;Peyrin-Biroulet L;Sandborn WJ
通讯作者：
Sandborn WJ
影响因子：
4.9
作者：
Vavricka SR;Schoepfer A;Scharl M;Lakatos PL;Navarini A;Rogler G
通讯作者：
Rogler G
中药联合治疗激素依赖性溃疡性结肠炎：随机对照试验研究方案
影响因子：
2.5
作者：
Zheng K;Shen H;Jia J;Lu Y;Zhu L;Zhang L;Shen Z
通讯作者：
Shen Z
影响因子：
--
作者：
Zhou MX;Wei X;Li AL;Wang AM;Lu LZ;Yang Y;Ren DM;Wang XN;Wen XS;Lou HX;Shen T
通讯作者：
Shen T